Determination N-Acetyl-L-Cysteine on Primesep SB Column

HPLC Method for N-Acetyl-L-cysteine on Primesep SB by SIELC Technologies

 

Condition 1
Column Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 20%
Buffer H3PO4 0.3%
Flow Rate 0.5 ml/min
Detection UV, 200 nm

 

Condition 2
Column Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 50%
Buffer Na2HPO4 pH 4.0 – 50 mM
Flow Rate 0.5 ml/min
Detection UV, 200 nm

High Performance Liquid Chromatography (HPLC) Method for Analysis of N-Acetyl-L-cysteine.

N-Acetyl-L-Cysteine (NAC), also known as Acetylcysteine, is the N-acetyl derivative of the naturally occurring amino acid L-cysteine with the chemical formula C5H9NO3S. It is primarily used to treat paracetamol overdoses, chronci bronchopulmonary disorders, and lactobezoar.

N-Acetyl-L-cysteine can be retained on Primesep SB, a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid (H3PO4) buffer. UV detection at 200 nm.

Description
Class of Compounds
 Nutritional supplement, Hydrophilic, Ionizable
Analyzing Compounds N-Acetyl-L-cysteine

 

Application Column

Primesep SB

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
N-Acetyl-L-cysteine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.