HPLC Method for Analysis of Pyrilamine, Acetaminophen, and Caffeine on Primesep SB Column

HPLC Method for Caffeine, Pyrilamine, Acetaminophen (Paracetamol) on Primesep SB by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Caffeine, Pyrilamine, Acetaminophen (Paracetamol)

Pyrilamine, also known as mepyramine, is a first-generation antihistamine with the molecular formula C17H23N3O. It works through targeting the H1 receptor and blocking effects of histamines. It can relieve allergy symptoms like sneezing, runny nose, and itchy, watery eyes. Due to it’s ability to cross the blood-brain carrier, it might have a strong drowsiness side-effect after ingestion. When used topically, it is used to treat skin reactions to bug bites, stings, nettles rashes, and more.

Acetaminophen, also known as Paracetamol, is a medication with the molecular formula C8H9NO2. It is an over-the-counter pain killer that also reduces fever. Unlike Ibuprofen and naproxen, it is not an anti-inflammatory drug.

Caffeine is a natural stimulant and methylxanthine alkaloid. with the molecular formula C6H10N4O2. Caffeine can be found in a variety of plants, including tea, coffea, cocoa, kola nuts, and guarana. Ingestion of it can increase alertness and cognitive function. It can also cause worsening anxiety, heart palpitations, and headaches

Caffeine, Pyrilamine, Acetaminophen (Paracetamol) can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN). Detection is performed using UV 310nm and LC MS ESI SIM 286.

You can find detailed UV spectra of Caffeine, Pyrilamine, Acetaminophen (Paracetamol) and information about its various lambda maxima by visiting the following link.

ColumnPrimesep SB, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeCN – 5% to 50%, 10 min
BufferSulfuric Acid
Flow Rate1.0 ml/min
DetectionUV 275
LOD* UV1-10 ppb, 2- 8 ppb, 3 – 2 ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsDrugs
Analyzing CompoundsCaffeine, Pyrilamine, Acetaminophen (Paracetamol)

Application Column

Primesep SB

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Acetaminophen (Paracetamol)
Caffeine
Pyrilamine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.