HPLC Method for Analysis of Neostigmine methylsulfate on Primesep 100 Column

HPLC Method for Neostigmine methylsulfate, Norneostigmine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Neostigmine methylsulfate, Norneostigmine.

Neostigmine methylsulfate is a drug with the chemical formula C13H22N2O6S. It is primarily used to reverse effects of non-depolarizing neuromuscular blocking agents after surgery. It works through inhibiting the enzyme acetylcholinesterase, preventing the breakdown of the neurotransmitter acetylcholine, which leads to a buildup of it at nerve endings, which stimulates muscle contractions.

Neostigmine methylsulfate, Norneostigmine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition
Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN – 30%
Buffer H2SO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 210 nm

Description
Class of Compounds Drug
Analyzing Compounds Neostigmine methylsulfate, Norneostigmine

 

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Neostigmine methylsulfate
Norneostigmine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.