ELSD HPLC Method for Analysis of Inorganic Ions on Chromni Column

HPLC Method for Chlorate, Nitrate, Bromate, Chloride, Sodium, Sulfate on Chromni by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Chlorate, Nitrate, Bromate, Chloride, Sodium, Sulfate.

Chlorate is a a compound with the chemical formula ClO3–. It is an oxidizer that works on Large variety of organics and other easily oxidized materials. Historically, it was used in pyrotechnics, but it faded out of use due to Chlorate’s instability. Magnesium chlorate has been found on Mars.

Nitrate, NO3–, is a compound of nitrogen and oxygen. It is essential as a nutrient in plants; therefore, it is often used in fertilizers. It is easily found in leafy greens. While it is important for cardiovascular health, too high of exposure to it can be dangerous.

Bromate is an oxyanion with the chemical formula BrO3–. It is formed in water through a reaction of ozone and bromide. It is suspected to be a human carcinogen.

Chloride is typically used to refer to a compound or molecule that contains a chlorine anion: Cl–. It is an electrolyte that is essential for bodily functions including blood pH, fluid balance, cellular functions, and more. Imbalance of chloride can indicate underlying health problems.

Sodium, which  has a chemical symbol of Na, is a soft alkali metal that is highly reactive.  It is found in abundance in everyday materials like table salt, sea water, and even the Earth’s crust. It is crucial  for the body’s function and fluid balance.

Sulfate is an ionic chemical with the formula SO42-. In nature, it is usually found in geodes like gypsum. It is also created in atmosphere from sulfur dioxide. It is used for a variety of applications, but most notably, coloring wood and creating light-reflecting paints.

Chlorate, Nitrate, Bromate, Chloride, Sodium, Sulfate can be retained and analyzed using the Chromni stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an Ammonium Acetate buffer. Detection is performed using MS.

ColumnChromni, 3.2 x 100 mm, 3 µm, 100 A, dual ended
Mobile PhaseGradient MeCN/H2O – 90/10-70/30% over 15 min
BufferAmmonium Acetate pH 5.0 – 10 mM
Flow Rate0.5 mL/min
DetectionELSD
Class of CompoundsIons
Analyzing CompoundsChlorate, Nitrate, Bromate, Chloride, Sodium, Sulfate

Application Column

Chromni

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Bromate
Chlorate
Chloride
Nitrate
Sodium
Sulfate
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.