HPLC Determination of Succinylcholine on Newcrom AH Column

HPLC Method for Succinylcholine chloride, Succinylcholine on Newcrom AH by SIELC Technologies

 

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of Succinylcholine

Succinylcholine chloride is a short-acting depolarizing neuromuscular blocker used as a short-term muscle relaxant. Succinylcholine is often used as an adjunct therapy in patients undergoing electroconvulsive shock therapy to control muscle contractions that are induced as a result of the electrical impulses delivered during the procedure.

By using a Newcrom AH mixed-mode column with a cation-exchange mechanism, Succinylcholine can be determinate in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and a perchloric acid (HClO4) buffer. UV detection at 200 nm.

Condition
Column Newcrom AH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 15/85%
Buffer  HClO4 – 0.85%
Flow Rate 1.0 ml/min
Detection UV, 200 nm

 

Description
Class of Compounds
 Drug
Analyzing Compounds Succinylcholine chloride, Succinylcholine

 

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Succinylcholine
Succinylcholine chloride
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.