High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Aminophenol, 4-Aminophenol hydrochloride l MS-compatible Mobile Phase on Primesep 100 by SIELC Technologies.
4-Aminophenol, also known as para-aminophenol, and Rodinal is a popular film developing compound and building block in organic chemistry reactions. Most notably, it is a key compound used in the production of acetaminophen (also known as paracetamol and Tylenol). It has the chemical formula H2NC6H4OH. It is usually found as a white, moderately soluble in alcohol, powder, but it can be recrystallized in hot water. You can find detailed UV spectra of 4-Aminophenol, 4-Aminophenol hydrochloride and information about its various lambda maxima by visiting the following link.
4-Aminophenol, 4-Aminophenol hydrochloride can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be detected in the UV regime at 275 nm, and due to the Ammonium Formate buffer, is also compatible with evaporative detectors such as Evaporative Light Scattering Detectors (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).
Condition
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 10% |
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Peak Retention Time | 3.7, 5.8 min |
Description
| Class of Compounds | Alcohols, Phenols, Amines, Amine Salts |
| Analyzing Compounds | 4-Aminophenol, 4-Aminophenol hydrochloride |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
4-Aminophenol hydrochloride
