| CAS Number | 51-78-5 |
|---|---|
| Molecular Formula | C6H8ClNO |
| Molecular Weight | 145.590 |
| InChI Key | RVGOBWDGAVAVPJ-UHFFFAOYSA-N |
| LogP | -0.00845 |
| Synonyms |
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Applications:
HPLC Method for Analysis of 4-Aminophenol on Primesep 100 Column
February 1, 2023
HPLC Method for Analysis of 4-Aminophenol on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analyses of 4-Aminophenol
4-Aminophenol (also known as para-aminophenol or Rodinal) is a popular film developing compound and building block in organic chemistry reactions. Most notably, it is a key compound used in the production of acetaminophen (also known as paracetamol or Tylenol). 4-Aminophenol can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an sulfuric acid (H2SO4) or Perchloric acid (HClO4)buffer. This analysis method can be detected in the UV regime at 275 nm.
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 – 0.2% or HClO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200, 210, 275 nm |
| Peak Retention Time | 3.7 min, 6.9 |
Description
| Class of Compounds | Alcohols, Phenols, Amines, Amine Salts |
| Analyzing Compounds | 4-Aminophenol |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options4-Aminophenol hydrochloride
HPLC Method for Analysis of 4-Aminophenol MS-compatible Mobile on Primesep 100 Column
December 9, 2022
High Performance Liquid Chromatography (HPLC) Method for Analysis of 4-Aminophenol MS-compatible Mobile Phase on Primesep 100 by SIELC Technologies.
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analyses of 4-Aminophenol
4-Aminophenol (also known as para-aminophenol or Rodinal) is a popular film developing compound and building block in organic chemistry reactions. Most notably, it is a key compound used in the production of acetaminophen (also known as paracetamol or Tylenol). 4-Aminophenol can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an Ammonium Formate (AmFm) buffer. This analysis method can be detected in the UV regime at 275 nm, and due to the Ammonium Formate buffer, is also compatible with evaporative detectors such as Evaporative Light Scattering Detectors (ELSD), Charged Aerosol Detection (CAD), and Electrospray Ionization (ESI) for Mass Spectrometry (MS).
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 10% |
| Buffer | Ammonium Formate pH 3.0 – 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Peak Retention Time | 3.7, 5.8 min |
Description
| Class of Compounds | Alcohols, Phenols, Amines, Amine Salts |
| Analyzing Compounds | 4-Aminophenol |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options4-Aminophenol hydrochloride
Separation of 4-Aminophenol hydrochloride on Newcrom R1 HPLC column
February 16, 2018
4-Aminophenol hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options