HPLC Method for Analysis of 4-Aminophenol on Primesep 100 by SIELC Technologies
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analyses of 4-Aminophenol
4-Aminophenol (also known as para-aminophenol or Rodinal) is a popular film developing compound and building block in organic chemistry reactions. Most notably, it is a key compound used in the production of acetaminophen (also known as paracetamol or Tylenol). 4-Aminophenol can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an sulfuric acid (H2SO4) or Perchloric acid (HClO4)buffer. This analysis method can be detected in the UV regime at 275 nm.
Condition
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 – 0.2% or HClO4 – 0.2% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200, 210, 275 nm |
| Peak Retention Time | 3.7 min, 6.9 |
Description
| Class of Compounds | Alcohols, Phenols, Amines, Amine Salts |
| Analyzing Compounds | 4-Aminophenol |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options4-Aminophenol hydrochloride
