HPLC Analysis of Caffeine in Drinks with Environmentally Friendly Mobile Phase on Primesep SB Column

HPLC Method for Caffeine on Primesep SB by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Caffeine.

Caffeine is a natural stimulant and methylxanthine alkaloid. with the molecular formula C6H10N4O2. Caffeine can be found in a variety of plants, including tea, coffea, cocoa, kola nuts, and guarana. Ingestion of it can increase alertness and cognitive function. It can also cause worsening anxiety, heart palpitations, and headaches.

Caffeine can be retained and analyzed using the Primesep SB stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and ethanol (EtOH) with a phosphoric acid buffer. Detection is performed using UV. You can find detailed UV spectra of Caffeine and information about its various lambda maxima by visiting the following link.

Condition
Column Primesep SB, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile Phase EtOH – 10%
Buffer H3PO4 – 0.3%
Flow Rate 0.5 ml/min
Detection UV, 270 nm

Description
Class of Compounds Xanthine, Hydrophobic, Ionizable
Analyzing Compounds Caffeine

 

Application Column

Primesep SB

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Caffeine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.