Gallic acid

Gallic acid structural formula

CAS Number149-91-7
Molecular FormulaC7H6O5
Molecular Weight170.120
InChI KeyLNTHITQWFMADLM-UHFFFAOYSA-N
LogP0.7
Synonyms
  • Gallic acid
  • 3,4,5-Trihydroxybenzoic acid
  • Benzoic acid, 3,4,5-trihydroxy-
  • 149-91-7
  • 3-10-00-02070
  • Benzoic acid, 3,4,5-trihydroxy-
  • 3,4,5-Trihydroxybenzoesaure
  • 3,4,5-trihydroxybenzoic acid
  • Acide 3,4,5-trihydroxybenzoique
  • acido 3,4,5-trihidroxibenzoico
  • GALLUSSAEURE
  • NSC 20103
  • NSC 674319
  • BRN 2050274
  • EINECS 205-749-9
  • Gallic acid, tech.
  • Kyselina 3,4,5-trihydroxybenzoova
  • Kyselina gallova
  • UNII-632XD903SP
  • 3,4,5-Trihydroxy-Benzoate
  • 3,4,5-Trihydroxy-Benzoic acid

Applications:

HPLC Method for Analysis of Gallic Acid in Bismuth Subgallate Tablets (Devrom)

May 5, 2022

Separation type: Liquid Chromatography Mixed-mode


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MS-compatible mobile phase


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High Performance Liquid Chromatography (HPLC) Method for Analysis of of Gallic Acid in Bismuth Subgallate Tablets

Gallic acid is a naturally-occuring phenolic acid that was a key ingredient in European iron gall ink from its first discovery in the 12th century until the 19th century. In modern times, it’s heavy metal salt, Bismuth subgallate, known by its trade name Devrom, is known for its effects at deodorizing flatulence and stools. Gallic acid, extracted from Devrom tablets, can be retained and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of water, Acetonitrile (MeCN), and Phosphoric acid (H3PO4). This analytical method can be UV detected at 200 nm with high resolution and peak symmetry.

 

Condition 1

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase MeCN – 10%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection UV 273nm

Condition 2

Column Newcrom BH, 3.2×100 mm,  100A
Mobile Phase MeCN – 10%
Buffer Formic acid – 0.05%
Flow Rate 1.0 ml/min
Detection UV 273nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds Gallic  Acid

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Bismuth subgallate
Gallic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Phenolic Acids

March 11, 2021


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Condition 1

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection 270 nm

Condition 2

Column Newcrom R1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection 270 nm

Description

Class of Compounds Acid
Analyzing Compounds Gallic acid, 3,4 Dihydroxybenzoic acid, Caffeic acid, trans-Ferulic acid, trans- p -Coumaric acid, trans-Cinnamic acid, Quercetin Hydrate, Salicylic acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
3,4-Dihydroxybenzoic Acid
Caffeic acid
Cinnamic acid
Ferulic acid
Gallic acid
Quercetin
Quercetin dihydrate
Salicylic acid
p-coumaric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Gallic Acid on Newcrom BH

January 27, 2021


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Gallic acid is a naturally occurring polyphenol compound present in fruits, vegetables, and herbals. Gallic acid has many biological properties, including antioxidant, anticancer, anti-inflammatory, and antimicrobial properties. Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, gallic acid can be retained and UV detected at 275nm.

Condition

Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection 275 nm

Description

Class of Compounds Acid
Analyzing Compounds Gallic acid

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Gallic acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Gallic acid on Newcrom R1 HPLC column

February 16, 2018
Separation of Gallic acid on Newcrom C18 HPLC column

Gallic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Gallic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.