HPLC Separation of Phenolic Acids


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Condition 1

Column Newcrom B, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection 270 nm

Condition 2

Column Newcrom R1, 4.6×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN
Buffer H3PO4 – 0.2%
Flow Rate 1.0 ml/min
Detection 270 nm

Description

Class of Compounds Acid
Analyzing Compounds Gallic acid, 3,4 Dihydroxybenzoic acid, Caffeic acid, trans-Ferulic acid, trans- p -Coumaric acid, trans-Cinnamic acid, Quercetin Hydrate, Salicylic acid

 

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
3,4-Dihydroxybenzoic Acid
Caffeic acid
Cinnamic acid
Ferulic acid
Gallic acid
Quercetin
Quercetin dihydrate
Salicylic acid
p-coumaric acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.