HPLC Determination of Gallic Acid on Newcrom BH

HPLC.cloud View on hplc.cloud Gallic acid is a naturally occurring polyphenol compound present in fruits, vegetables, and herbals. Gallic acid has many biological properties, including antioxidant, anticancer, anti-inflammatory, and antimicrobial properties. Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, gallic acid can be retained and UV detected at 275nm.


Column Newcrom BH, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 10/90%
Buffer H3PO4 - 0.5%
Flow Rate 1.0 ml/min
Detection 275 nm


Class of Compounds Acid
Analyzing Compounds Gallic Acid
Application Analytes:
Gallic acid