HPLC Method for Analysis of Gallic Acid on Newcrom BH

HPLC Method for Gallic acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Gallic acid.

Gallic acid is a naturally occurring polyphenol compound present in fruits, vegetables, and herbals with the chemical formula C7H6O5. Gallic acid has many biological properties, including antioxidant, anticancer, anti-inflammatory, and antimicrobial properties. It was a key ingredient in European iron gall ink from its first discovery in the 12th century until the 19th century. In modern times, it’s heavy metal salt, Bismuth subgallate, known by its trade name Devrom, is known for its effects at deodorizing flatulence and stools.

Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, Gallic acid can be retained and UV detected at 275nm.

Condition
Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection 275 nm

Description
Class of Compounds Acid
Analyzing Compounds Gallic acid

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Gallic acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.