HPLC Determination of Gallic Acid on Newcrom BH

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Gallic acid is a naturally occurring polyphenol compound present in fruits, vegetables, and herbals. Gallic acid has many biological properties, including antioxidant, anticancer, anti-inflammatory, and antimicrobial properties. Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, gallic acid can be retained and UV detected at 275nm.


Column Newcrom BH, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 10/90%
Buffer H3PO4 – 0.5%
Flow Rate 1.0 ml/min
Detection 275 nm


Class of Compounds Acid
Analyzing Compounds Gallic Acid


Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Gallic acid
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