Gallic acid is a naturally occurring polyphenol compound present in fruits, vegetables, and herbals. Gallic acid has many biological properties, including antioxidant, anticancer, anti-inflammatory, and antimicrobial properties. Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, gallic acid can be retained and UV detected at 275nm.
Column | Newcrom BH, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 10/90% |
Buffer | H3PO4 – 0.5% |
Flow Rate | 1.0 ml/min |
Detection | 275 nm |
Class of Compounds | Acid |
Analyzing Compounds | Gallic Acid |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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