Gallic acid is a naturally occurring polyphenol compound present in fruits, vegetables, and herbals. Gallic acid has many biological properties, including antioxidant, anticancer, anti-inflammatory, and antimicrobial properties. Using Newcrom BH mixed-mode column and a mobile phase consisting of acetonitrile (ACN), and water with phosphoric acid (H3PO4) buffer, gallic acid can be retained and UV detected at 275nm.
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 10/90% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | 275 nm |
| Class of Compounds | Acid |
| Analyzing Compounds | Gallic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
Gallic acidApplication Detection:
UV Detection
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