| CAS Number | 99-26-3 |
|---|---|
| Molecular Formula | C7H5BiO6 |
| Molecular Weight | 394.092 |
| InChI Key | JAONZGLTYYUPCT-UHFFFAOYSA-K |
| LogP | 0.81 |
| Synonyms |
|
Applications:
HPLC UV Method for Analysis of Gallic Acid in Bismuth Subgallate Tablets (Devrom) on Newcrom BH Column
May 5, 2022
HPLC Method for Bismuth subgallate, Gallic acid on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bismuth subgallate, Gallic acid.
Gallic acid is a naturally-occurring phenolic acid with the chemical formula the chemical formula C7H6O5. It can be found in gallnuts, sumac, witch hazel, tea leaves, oak bark, and other plants. Historically, it was a key ingredient in European iron gall ink. The use of it spanned from its first discovery in the 12th century into the 19th century. In the modern times, it is used as an antioxidant and a preservative. You can find detailed UV spectra of Gallic acid and information about its various lambda maxima by visiting the following link.
Bismuth subgallate is a medication with the chemical formula C7H5BiO6. It is primarily used to deodorize flatulence and stools. Additionally, it is used to treat the Helicobacter pylori infection.
Bismuth subgallate, Gallic acid can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid buffer. Detection is performed using UV.
| Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 10% |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 273nm |
| Class of Compounds | Acid, Hydrophilic |
| Analyzing Compounds | Bismuth subgallate, Gallic acid |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Gallic acid
Separation of Bismuth subgallate on Newcrom R1 HPLC column
February 16, 2018
Bismuth subgallate can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options