| CAS Number | 60142-96-3 |
|---|---|
| Molecular Formula | C9H17NO2 |
| Molecular Weight | 171.241 |
| InChI Key | UGJMXCAKCUNAIE-UHFFFAOYSA-N |
| LogP | -1.10 |
| Synonyms |
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Applications:
HPLC Determination of Gamma-aminobutyric Acid (GABA), Gabapentin and Pregabalin on Primesep N Column
June 8, 2021
HPLC Method for gamma-Aminobutyric Acid (GABA), Gabapentin, Gabapentin hydrochloride, Pregabalin on Primesep N by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of GABA, Gabapentin and Pregabalin
Gamma-Aminobutyric Acid (GABA) is a neurotransmitter with the chemical formula C4H9NO2. It is crucial in the nervous system for reducing anxiety and promoting sleep. It is naturally produced in the body, but can also be found in supplements and some fermented foods.
Pregabalin is a medication with the chemical formula C8H17NO2. It is an anticonvulsant, analgesic, and anxiolytic amino acid that is used to treat epilepsy, restless leg syndrome, anxiety, neuropathic pain, and fibromyalgia. In the US it is used for adjunctive therapy for adults, while in the European Union, United Kingdom, and Russia, it is approved for treatment of generalized anxiety.
Gabapentin is a prescription medication with the chemical formula C9H17NO2. It is used to treat epilepsy, neuropathic pain, hot flashes, and restless legs syndrome. Occasionally, Gabapentin is also used to treat anxiety and sleep disorders.
| Column | Primesep N, 4.6 x 150 mm, 5 µm, 300 A, dual ended |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 – 10 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) (MS-compatible mobile phase) |
| Class of Compounds |
Acid, Drug |
| Analyzing Compounds | gamma-Aminobutyric Acid (GABA), Gabapentin, Gabapentin hydrochloride, Pregabalin |
Application Column
Primesep N
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 300 A
Column options: dual ended
Gabapentin hydrochloride
Pregabalin
gamma-Aminobutyric Acid (GABA)
HPLC Method for Analysis of Gabapentin Tablets
June 11, 2018
HPLC Method for Gabapentin, Gabapentin hydrochloride on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Gabapentin, Gabapentin hydrochloride
Gabapentin is a drug used to treat epilepsy, neuropathic pain, hot flashes, and restless legs syndrome. It also is used to treat neuropathic pain. Gabapentin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It is also suitable for pharmacokinetics.
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 1.0 ml/min |
| Detection | CAD (Corona) MS- compatible mobile phase |
| Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.05 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 210 nm |
| Class of Compounds |
Drug, Nerve pain medication, Anticonvulsant, Hydrophobic, Ionizable, Zwitterionic |
| Analyzing Compounds | Gabapentin, Gabapentin hydrochloride |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Gabapentin hydrochloride
CAD Detection
