HPLC Method for Analysis of Gabapentin Tablets

HPLC Method for Gabapentin, Gabapentin hydrochloride on Primesep 100 by SIELC Technologies

HPLC Method for Analysis of Gabapentin Tablets

High Performance Liquid Chromatography (HPLC) Method for Analysis of Gabapentin, Gabapentin hydrochloride 

Gabapentin is a drug used to treat epilepsy, neuropathic pain, hot flashes, and restless legs syndrome. It also is used to treat neuropathic pain. Gabapentin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particle columns are available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation of impurities in preparative separation. It is also suitable for pharmacokinetics.

Condition 1

Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 30/70%
Buffer AmFm pH 3.0 – 30 mM
Flow Rate 1.0 ml/min
Detection CAD (Corona) MS- compatible mobile phase

Condition 2

Column Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase MeCN/H2O – 50/50%
Buffer H2SO4 – 0.05 %
Flow Rate 1.0 ml/min
Detection UV, 210 nm

Description

Class of Compounds
Drug,  Nerve pain medication, Anticonvulsant, Hydrophobic, Ionizable, Zwitterionic
Analyzing Compounds Gabapentin, Gabapentin hydrochloride

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Gabapentin
Gabapentin hydrochloride

Application Detection:
UV Detection
CAD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.