Diclofenac

Diclofenac structural formula

CAS Number15307-86-5
Molecular FormulaC14H11Cl2NO2
Molecular Weight296.150
InChI KeyDCOPUUMXTXDBNB-UHFFFAOYSA-N
LogP4.51
Synonyms
  • Diclofenac
  • [2-(2,6-Dichloroanilino)phenyl]acetic acid
  • Benzeneacetic acid, 2-[(2,6-dichlorophenyl)amino]-
  • 15307-86-5
  • Benzeneacetic acid, 2-[(2,6-dichlorophenyl)amino]-
  • [o-(2,6-Dichloroanilino)phenyl]acetic acid
  • 2-(2,6-Dichloroanilino)phenylacetic acid
  • 2-(2,6-Dichlorophenylamino)phenylacetic acid
  • 2-[(2,6-Dichlorophenyl)amino]benzeneacetic acid
  • Acetic acid, [o-(2,6-dichloroanilino)phenyl]-
  • Dichlofenac
  • Diclofenac acid
  • diclofenaco
  • Diclofenamic acid
  • Diclomelan
  • Dicloreuma
  • N-(2,6-Dichlorophenyl)-o-aminophenylacetic acid
  • Pennsaid
  • Transfenac
  • BRN 2146636
  • 2-((2,6-Dichlorophenyl)amino)benzeneacetic acid
  • Diclophenac
  • EINECS 239-348-5
  • ProSorb-D
  • Diclofenacum
  • UNII-144O8QL0L1
  • Zorovolex

Applications:

HPLC Method for Analysis of Diclofenac and Carbamazepine on Primesep 100 Column

July 17, 2025

HPLC Method for Diclofenac, Carbamazepine on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Diclofenac, Carbamazepine

Diclofenac is an organic compound with the molecular formula C14H11Cl2NO2.

Properties:
Appearance: Typically an odorless, white to off-white crystalline, slightly hygroscopic powder.

Molecular weight: ~296.1 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses:  A nonsteroidal anti-inflammatory drug (NSAID) used to relieve pain and reduce inflammation and swelling.

Carbamazepine is an organic compound with the molecular formula C15H12N2O.

Properties:
Appearance: Typically a white to off-white crystalline powder.

Molecular weight: ~236.27 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses: Mainly used to treat several conditions affecting the nervous system such as epilepsy.

Diclofenac, Carbamazepine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 220 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 45%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 220 nm

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsDrug
Analyzing CompoundsDiclofenac, Carbamazepine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Carbamazepine
Diclofenac
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

UV-Vis Spectrum of Diclofenac

July 16, 2025
UV-Vis Spectrum of Diclofenac. Absorption Maxima: 200 nm, 276 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Diclofenac
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Diclofenac

May 6, 2016

Chr_1065




Diclofenac non-steroidal anti-inflammatory agent (NSAID) with antipyretic and analgesic actions. It is used to relieve pain, swelling (inflammation), and joint stiffness. As side effects, it can cause nausea, heartburn, constipation, headache, drowsiness, and dizziness. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Diclofenac. The method is UV compatible and can be used as a general approach for analyzing similar compounds.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Diclofenac
Diclofenac sodium

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.