HPLC Method for Diclofenac, Carbamazepine on Primesep 100 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Diclofenac, Carbamazepine

Diclofenac is an organic compound with the molecular formula C₁₄H₁₁Cl₂NO₂.

Properties:
Appearance: Typically an odorless, white to off-white crystalline, slightly hygroscopic powder.

Molecular weight: ~296.1 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses:  A nonsteroidal anti-inflammatory drug (NSAID) used to relieve pain and reduce inflammation and swelling.

Carbamazepine is an organic compound with the molecular formula C₁₅H₁₂N₂O.

Properties:
Appearance: Typically a white to off-white crystalline powder.

Molecular weight: ~236.27 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses: Mainly used to treat several conditions affecting the nervous system such as epilepsy.

Diclofenac, Carbamazepine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 220 nm.

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Diclofenac non-steroidal anti-inflammatory agent (NSAID) with antipyretic and analgesic actions. It is used to relieve pain, swelling (inflammation), and joint stiffness. As side effects, it can cause nausea, heartburn, constipation, headache, drowsiness, and dizziness. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Diclofenac. The method is UV compatible and can be used as a general approach for analyzing similar compounds.