Carbamazepine

Carbamazepine structural formula

CAS Number298-46-4
Molecular FormulaC15H12N2O
Molecular Weight236.275
InChI KeyFFGPTBGBLSHEPO-UHFFFAOYSA-N
LogP2.45
Synonyms
  • Carbamazepine
  • 5H-Dibenzo[b,f]azepine-5-carboxamide
  • 5H-Dibenz[b,f]azepine-5-carboxamide
  • 298-46-4
  • Carbazepine
  • 5H-Dibenz[b,f]azepine-5-carboxamide
  • 5-Carbamoyl-5H-dibenz[b,f]azepine
  • 5H-Dibenzo [b,f] azepine-5-carboxamide
  • Amizepin
  • Calepsin
  • Carbamazepen
  • Carbamazepin
  • carbamazepina
  • Carbatrol
  • Carbelan
  • Finlepsin
  • Geigy 32883
  • Karbamazepin
  • Karbelex
  • Karberol
  • Neurotol
  • Neurotop
  • NSC 169864
  • Stazepine
  • Tegretal
  • Tegretol
  • Tegretol XR
  • Telesmin
  • Timonil
  • BRN 1246090
  • Carbamezepine
  • 5-Carbamoyl-5H-dibenz(b,f)azepine
  • EINECS 206-062-7
  • Bipotrol
  • Equetro
  • Tegretol-XR
  • Carbamazepinum
  • UNII-33CM23913M
  • Carbatrol extended-release
  • 5-Carbamoyl-5H-dibenzo(b,f)azepine
  • 5-Carbamyl-5H-dibenzo(b,f)azepine
  • 5H-Dibenz(b,f)azepine-5-carboxamide
  • Carbamazepine

Applications:

HPLC Method for Analysis of Carbamazepine on Primesep 100 Column

July 17, 2025

HPLC Method for Carbamazepine on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Carbamazepine

Carbamazepine is an organic compound with the molecular formula C15H12N2O.

Properties:
Appearance: Typically a white to off-white crystalline powder.

Molecular weight: ~236.27 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses: Mainly used to treat several conditions affecting the nervous system such as epilepsy.

Carbamazepine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 212 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 35%
BufferH2SO4 – 0.2%
Flow Rate1.0 ml/min
DetectionUV 212 nm
LOD5 ppb

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsDrug
Analyzing CompoundsCarbamazepine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Carbamazepine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Diclofenac and Carbamazepine on Primesep 100 Column

July 17, 2025

HPLC Method for Diclofenac, Carbamazepine on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Diclofenac, Carbamazepine

Diclofenac is an organic compound with the molecular formula C14H11Cl2NO2.

Properties:
Appearance: Typically an odorless, white to off-white crystalline, slightly hygroscopic powder.

Molecular weight: ~296.1 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses:  A nonsteroidal anti-inflammatory drug (NSAID) used to relieve pain and reduce inflammation and swelling.

Carbamazepine is an organic compound with the molecular formula C15H12N2O.

Properties:
Appearance: Typically a white to off-white crystalline powder.

Molecular weight: ~236.27 g/mol

Solubility: Soluble in organic solvents like ethanol.

Uses: Mainly used to treat several conditions affecting the nervous system such as epilepsy.

Diclofenac, Carbamazepine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 220 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 45%
BufferH2SO4 – 0.1%
Flow Rate1.0 ml/min
DetectionUV 220 nm

*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of CompoundsDrug
Analyzing CompoundsDiclofenac, Carbamazepine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

Add to cart
Application Analytes:
Carbamazepine
Diclofenac
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

UV-Vis Spectrum of Carbamazepine

July 16, 2025
UV-Vis Spectrum of Carbamazepine. Absorption Maxima: 192 nm, 212 nm, 235 nm, 286 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Carbamazepine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Carbamazepine on Newcrom R1 HPLC column

February 16, 2018
Separation of Carbamazepine on Newcrom C18 HPLC column

Carbamazepine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Carbamazepine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.