If you are looking for optimized HPLC method to analyze Sulfamethoxazole check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Trimethoprim, Sulfamethoxazole on Newcrom R1 by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Trimethoprim, Sulfamethoxazole.

Sulfamethoxazole, also written as SMZ and SMX, is an antibiotic with the chemical formula C₁₀H₁₁N₃O₃S.  It is used primarily to treat bacterial infections including but not limited to bronchitis, prostatitis, urinary tract infections, as well as gam negative and positive bacteria. You can find detailed UV spectra of Sulfamethoxazole and information about its various lambda maxima by visiting the following link.

Trimethoprim, also written as TMP, is an antibiotic with the chemical formula C₁₄H₁₈N₄O₃. It is used primarily to treat bladder infections as well as middle ear infections and travelers’ diarrhea. People with HIV/AIDS might take it to treat Pneumocystis pneumonia. It works through binding to dihydrofolate reductase and inhibiting the production of dihydrofolic and tetrahydrofolic acid. You can find detailed UV spectra of Trimethoprim and information about its various lambda maxima by visiting the following link.

Trimethoprim, Sulfamethoxazole can be retained and analyzed using the Newcrom R1 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).