| CAS Number | 3416-24-8 |
|---|---|
| Molecular Formula | C6H13NO5 |
| Molecular Weight | 179.172 |
| InChI Key | MSWZFWKMSRAUBD-QZABAPFNSA-N |
| LogP | -2.34 |
| Synonyms |
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Applications:
HPLC Retention of Glucosamine
August 22, 2008
Glucosamine is an amino sugar that is used as precursor in synthesis of glycosylated proteins and peptides. Glucosamine is also a component of several medications, example being oral glucosamine being is used to treat arthritis. It is a very polar compound with no UV activity and pKa of 11. Compound is not retained on modern HPLC columns without ion-pairing reagents. It is often required to quantitate glucosamine in complex matrices with LC/MS detection, making the use of ion-pairing reagents impossible. In this HPLC application glucosamine is retained by cation-exchange mechanism on Primesep 100 mixed-mode column. Retention of glucosamine is controlled by buffer nature, buffer concentration and buffer pH. Method can be used for fast determination of glucosamine in mixtures with proteins, sugars and sugar phosphates. ELSD and LC/MS are detection of choice for polar non-UV active compounds.
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column
October 4, 2007
The Separation of Phenylalanine, Glucosamine and Tryptophan demonstrates how the small changes to the organic and/or buffer concentrations in the mobile phase affect the retention of compounds on a mixed-mode column by hydrophobic, ion-exchange and ion-exclusion mechanisms in reverse-phase HPLC chromatography. Evaporative Light Scattering Detector (ELSD) used
| Column | Primesep 100, 2.1×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm |
| Flow Rate | 0.25 ml/min |
| Detection | ELSD |
| Class of Compounds |
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Phenylalanine, Glucosamine, Tryptophan |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPhenylalanine
Tryptophan
HPLC Separation of Glucosamine from Chondroitin Sulfate
March 27, 2006
Glucosamine is an amino sugar believed to play a role in cartilage formation and repair. Chondroitin sultate gives cartilage elasticity. Primesep 500 separates glucosamine and chondroitin sulfate with baseline resolution. Chondroitin sulfate is separated from chloride ion by ion exclusion and glucosamine is separated by sodium ion by ion exchange and reversed phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and ammonium formate with evaporative light scattering detection (ELSD) 90C.
Application Column
Primesep 500
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsGlucosamine
