Glucosamine

Glucosamine structural formula

CAS Number3416-24-8
Molecular FormulaC6H13NO5
Molecular Weight179.172
InChI KeyMSWZFWKMSRAUBD-QZABAPFNSA-N
LogP-2.34
Synonyms
  • Glucosamine
  • 2-Amino-2-deoxy-beta-D-glucopyranose
  • beta-D-Glucopyranose, 2-amino-2-deoxy-
  • 3416-24-8
  • 4-04-00-02017
  • D-Glucose, 2-amino-2-deoxy-
  • 2-Amino-2-deoxy-D-glucopyranose
  • 2-Amino-2-deoxy-D-glucose
  • 2-Amino-2-deoxyglucose
  • 2-Aminoglucose
  • 2-Deoxy-2-amino-D-glucose
  • 2-Deoxy-2-aminoglucose
  • Chitosamine
  • D-Glucosamine
  • Glucosamin
  • glucosamina
  • Glucosamine, D-
  • BRN 1724602
  • EINECS 222-311-2
  • Glucosaminum
  • UNII-N08U5BOQ1K
  • 1261161-52-7
  • 149014-32-4
  • 2351-15-7
  • 58-87-7
  • 58267-75-7
  • 880765-44-6
  • 911653-84-4

Applications:

HPLC Retention of Glucosamine

August 22, 2008

Glucosamine is an amino sugar that is used as precursor in synthesis of glycosylated proteins and peptides. Glucosamine is also a component of several medications, example being oral glucosamine being is used to treat arthritis. It is a very polar compound with no UV activity and pKa of 11. Compound is not retained on modern HPLC columns without ion-pairing reagents. It is often required to quantitate glucosamine in complex matrices with LC/MS detection, making the use of ion-pairing reagents impossible. In this HPLC application glucosamine is retained by cation-exchange mechanism on Primesep 100 mixed-mode column. Retention of glucosamine is controlled by buffer nature, buffer concentration and buffer pH. Method can be used for fast determination of glucosamine in mixtures with proteins, sugars and sugar phosphates. ELSD and LC/MS are detection of choice for polar non-UV active compounds.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Glucosamine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Phenylalanine, Glucosamine, and Tryptophan on Mixed-Mode Column

October 4, 2007


The Separation of Phenylalanine, Glucosamine and Tryptophan demonstrates how the small changes to the organic and/or buffer concentrations in the mobile phase affect the retention of compounds on a mixed-mode column by hydrophobic, ion-exchange and ion-exclusion mechanisms in reverse-phase HPLC chromatography. Evaporative Light Scattering Detector (ELSD) used

Condition

Column Primesep 100, 2.1×100 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm
Flow Rate 0.25 ml/min
Detection ELSD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Phenylalanine, Glucosamine, Tryptophan

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Glucosamine
Phenylalanine
Tryptophan

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Glucosamine from Chondroitin Sulfate

March 27, 2006

Glucosamine is an amino sugar believed to play a role in cartilage formation and repair. Chondroitin sultate gives cartilage elasticity. Primesep 500 separates glucosamine and chondroitin sulfate with baseline resolution. Chondroitin sulfate is separated from chloride ion by ion exclusion and glucosamine is separated by sodium ion by ion exchange and reversed phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and ammonium formate with evaporative light scattering detection (ELSD) 90C.

Application Column

Primesep 500

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Chondroitin Sulfate
Glucosamine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.