| CAS Number | 80-08-0 |
|---|---|
| Molecular Formula | C12H12N2O2S |
| Molecular Weight | 248.301 |
| InChI Key | MQJKPEGWNLWLTK-UHFFFAOYSA-N |
| LogP | 0.97 |
| Synonyms |
|
Applications:
HPLC Separation of Antibiotics on Newcrom A Column
June 11, 2020
Antibiotics are widely used for treatment and prevention of bacterial infections. Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups. Methanol can be used in the mobile phase on Newcrom A column. The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer. UV detection at 275nm.
| Column | Newcrom A, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeOH Gradient |
| Buffer | H2SO4 Gradient |
| Flow Rate | 0.5 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Drugs, Antibiotics |
| Analyzing Compounds | Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline |
Application Column
Newcrom A
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Sulfadiazine
Sulfaguanidine
Sulfanilamide
Sulphaquinoxaline
HPLC Separation of Antibiotics on Primesep 200 Column
June 6, 2020
Antibiotics are widely used for treatment and prevention of bacterial infections. Various antibiotics, particularly those with a sulfanilamide structure, were separated in HPLC using mixed-mode columns with varying strengths of ion-pairing groups. Primesep 200 has weak acidic ion-exchange pairing groups while Newcrom A has strong acidic ion-exchange groups. In addition, methanol can be used in the mobile phase on Newcrom A column. The antibiotics were resolved on both columns with a gradient mobile phase consisting of acetonitrile (ACN) or methanol (MeOH), water and sulfuric acid (H2SO4) buffer. UV detection at 275nm.
| Column | Primesep 200, 4.6 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN Gradient |
| Buffer | H2SO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 275 nm |
| Class of Compounds | Drugs, Antibiotics |
| Analyzing Compounds | Sulfanilamide, Sulfadiazine, Sulfaguanidine, Dapsone, Sulfaquinoxaline |
Application Column
Primesep 200
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Sulfadiazine
Sulfaguanidine
Sulfanilamide
Analysis of Antibiotic Dapsone and Related Impurities in Mixed-Mode Chromatography
May 12, 2015
Separation type: Liquid Chromatography Mixed-mode
Dapsone is an antibiotic used to treat leprosy and acne. Obelisc R was used to analyze dapsone and its impurities. Obelisc R is a tri-modal reversed-phase, cation- and anion-exchange column. Method is compatible with LC/MS and prep chromatography.
| Column | Obelisc R, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 0-25%, 6 min, 25-70% 14 min |
| Buffer | Gradient Formic Acid – 0.05%-0.3%, 10 min, 14 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | Dapsone |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsUV Detection
Separation of Antibiotics in Mixed-mode chromatography
May 11, 2015
A complex mixture of sulphonamide, macrolide, tetracycline and fluoroquinolone antibiotics were separated in one run using mixed-mode chromatography with LC/MS -compatible conditions. All compounds are separated based on reversed-phase and/or ion-exchange mechanism. Method can be used for analysis of various classes of antibiotics and related impurities in different sample matrices (blood, urine, soil, waste water).
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 0-25%, 6 min, 25-70% 14 min |
| Buffer | Gradient Formic Acid – 0.05%-0.3%, 10 min, 14 min hold |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Antibiotic, Drug, Hydrophobic, Ionizable |
| Analyzing Compounds | Sulphanilamide, Sulphaguanidine, Oxytetracycline, Marbofloxacin, Sulphadiazine, Chlortetracycline, Nofloxacin, Spiramycin, Cefalonium, Tylosin, Josamycin, Sulphamethoxazole, Dapsone, Sulphaquinoxalline, Flumequine |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsChlortetracycline
Dapsone
Flumequine
Josamycin
Marbofloxacin
Norfloxacin
Oxytetracycline
Spiramycin
Sulfamethoxazole
Sulfonamides
Sulphadiazine
Sulphaguanidine
Sulphanilamide
Sulphaquinoxaline
Tetracycline
Tylosin
UV Detection
