| CAS Number | 90-41-5 |
|---|---|
| Molecular Formula | C12H11N |
| Molecular Weight | 169.228 |
| InChI Key | TWBPWBPGNQWFSJ-UHFFFAOYSA-N |
| LogP | 2.84 |
| Synonyms |
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Applications:
Separation of Toluidine and Aminobiphenyl Isomers
July 6, 2015
Toluidine isomers and aminobyphenyl isomers are organic compounds each with a substituted amine group. Primesep 200 was used to separate these isomers. Primesep 200 separates isomers by interacting with polar molecules, retaining basic compounds, and additionally by reverse phase mechanism.
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm Ph 3.5 |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Hydrophobic, Ionizable |
| Analyzing Compounds | o-Toluidine, 2-Aminobiphenyl, m-Toluidine, p-Toluidine, 4-Aminobiphenyl |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options4-Aminobiphenyl
m-Toluidine
o-Toluidine
p-Toluidine
HPLC Separation of Aminobiphenyl Isomers in Cation-Exchange Mode
July 14, 2011
Two isomers of aminobiphenyl were successfully retained and separated in cation-exchange mode on a Primesep S2 column. Primesep S2 column is a HILIC/cation-exchange column that can be operated in HILIC, cation-exchange, and anion-exclusion modes. Method and column are compatible with ELSD/LC/MS and UV and can be used for routine analysis of polar molecules.
| Column | Primesep S2, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 5/95% |
| Buffer | AmAc pH 5.0 15 mM |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Hydrophobic, Ionizable |
| Analyzing Compounds | 2-Aminobiphenyl, 4 -Aminobiphenyl |
Application Column
Primesep S2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options4-Aminobiphenyl
HPLC Separation of Aminobiphenyls
April 10, 2005
Aminobiphenyls consist of isomers that differ only in the location of a primary amine group on an aromatic ring. 2-, 3-, and 4-aminobiphenyl are separated on a Primesep D with good peak shape and a short retention time by a mixture of reversed-phase and ion-exclusion interactions. Peak order can be reversed for 2- and 3-aminobiphenyls by using ammonium formate buffer without pH adjustment. The HPLC separations use a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate buffer or acetic acid (HOAc) with UV detection at 250 nm.
| Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | Formic Acid, AmFm |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Hydrophobic, Ionizable |
| Analyzing Compounds | 2-Aminobiphenyl, 4 -Aminobiphenyl, 3 -Aminobiphenyl |
Application Column
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options3-Aminobiphenyl
4-Aminobiphenyl
