HPLC Separation of Aminobiphenyl Isomers in Cation-Exchange Mode

chr_308.gif Two isomers of aminobiphenyl were successfully retained and separated in cation-exchange mode on a Primesep S2 column. Primesep S2 column is a HILIC/cation-exchange column that can be operated in HILIC, cation-exchange, and anion-exclusion modes. Method and column are compatible with ELSD/LC/MS and UV and can be used for routine analysis of polar molecules.


Column Primesep S2, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 5/95%
Buffer AmAc pH 5.0 15 mM
Flow Rate 1.0 ml/min
Detection UV, 270 nm


Class of Compounds Hydrophobic, Ionizable
Analyzing Compounds 2-Aminobiphenyl, 4 -Aminobiphenyl

Application Analytes: