Separation of Toluidine and Aminobiphenyl Isomers

Toluidine isomers and aminobyphenyl isomers are organic compounds each with a substituted amine group. Primesep 200 was used to separate these isomers. Primesep 200 separates isomers by interacting with polar molecules, retaining basic compounds, and additionally by reverse phase mechanism.


Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm Ph 3.5
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds Hydrophobic, Ionizable
Analyzing Compounds o-Toluidine, 2-Aminobiphenyl, m-Toluidine, p-Toluidine, 4-Aminobiphenyl
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Application Analytes:

Application Detection:
ELSD/MS Detection