| CAS Number | 66722-44-9 |
|---|---|
| Molecular Formula | C18H31NO4 |
| Molecular Weight | 325.450 |
| InChI Key | VHYCDWMUTMEGQY-UHFFFAOYSA-N |
| LogP | 1.87 |
| Synonyms |
|
Applications:
Uv-Vis Spectrum of Bisoprolol
September 29, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Bisoprolol check our HPLC Applications library
UV/Vis.: λmax: 194 nm, 225 nm, 270 nm
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This compound was diluted in a mixture of water and acetonitrile. The method was done with a mixture of water and acetonitrile.
HPLC MS Method for Analysis of Bisoprolol in Pharmaceutical Formulation on Primesep 100 Column
September 24, 2026
HPLC Method for Analysis of Bisoprolol on Primesep 100 by SIELC Technologies
Bisoprolol is a beta-blocker with the chemical formula C18H31NO4. It is used primarily used to treat high blood pressure, heart failure, and chest pain. It works through targeting receptors, specifically epinephrine, in the heart, which slows heart rate and lowers blood pressure.
Bisoprolol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and Ammonium Formate. Detection is performed using LC MS.
Condition
| Column | Primesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/H2O – 60/40% |
| Buffer | Ammonium formate pH 3.0 – 40 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV 272 nm, LC MS ESI-SIM 326 (M+H)+ |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Bisoprolol |
Application Column
Primesep 100
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
HPLC Chiral Separation of Bisoprolol Enantiomers on Cyralis CM Column
September 23, 2026
HPLC Chiral Method for Analysis of Bisoprolol Enantiomers on Cyralis CM Column by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Bisoprolol.
Bisoprolol is a beta-blocker with the chemical formula C18H31NO4. It is used primarily used to treat high blood pressure, heart failure, and chest pain. It works through targeting receptors, specifically epinephrine, in the heart, which slows heart rate and lowers blood pressure.
This application describes a normal-phase chiral HPLC method for the separation of Bisoprolol enantiomers using SIELC’s Cyralis CM chiral column. Cyralis CM is a cellulose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications. For best results, the sample should be prepared fresh for analysis.
Bisoprolol can be retained and analyzed using the Cyralis CM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of sodium Hexane, Ethanol, and DEA. Detection is performed using UV.
Condition
| Column | Cyralis CM, 4.6 x 100 mm, 5 µm, 100 A, normal phase |
| Mobile Phase | Hexane/Ethanol/DEA |
| Flow Rate | 1.0 mL/min |
| Detection | UV 230 nm |
| Resolution | 1.98 |
Description
| Class of Compounds | Drug |
| Analyzing Compounds | Bisoprolol |
Application Column
Cyralis CM
Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase
Separation of Bisoprolol on Newcrom R1 HPLC column
February 16, 2018
Bisoprolol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options