HPLC MS Method for Analysis of Bisoprolol in Pharmaceutical Formulation on Primesep 100 Column

HPLC Method for Analysis of Bisoprolol on Primesep NC by SIELC Technologies

HPLC Method for Analysis of Bisoprolol in on Primesep 100 Column

Bisoprolol is a beta-blocker with the chemical formula C18H31NO4. It is used primarily used to treat high blood pressure, heart failure, and chest pain. It works through targeting receptors, specifically epinephrine, in the heart, which slows heart rate and lowers blood pressure.

Bisoprolol can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of acetonitrile, water, and Ammonium Formate. Detection is performed using LC MS.

Condition

ColumnPrimesep 100, 3.2 x 100 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/H2O – 60/40%
BufferAmmonium formate pH 3.0 – 40 mM
Flow Rate0.5 ml/min
DetectionUV 272 nm, LC MS ESI-SIM 326 (M+H)+

Description

Class of CompoundsDrug
Analyzing CompoundsBisoprolol

Application Column

Primesep 100

Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Bisoprolol
Application Detection:
LC MS Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.