HPLC Method for Analysis of beta-Carotene, Lycopene on Lipak Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of beta-Carotene, Lycopene.

Lycopene is a carotenoid hydrocarbon with C₄₀H₅₆ molecular formula. It is found primarily in tomatoes, watermelon, grapefruits, and red guavas. Gac has the highest concentration of lycopene at 2~6 per gram. Lycopene in non-toxic, but can be a rare allergen. You can find detailed UV spectra of Lycopene and information about its various lambda maxima by visiting the following link.

beta-Carotene, also written as β-Carotene, is a pigment with the chemical formula C₄₀H₅₆. It is found primarily in fungi, plants, and fruit. Especially high amounts of it are found in carrots, pumpkins, spinach, and sweet potatoes. In dietary contexts, it is used as a pro-vitamin, as it converts to retinol in the body. In fact, it is the worldwide primary dietary source of provitamin A and is the best known provitamin a carotenoid. You can find detailed UV spectra of beta-Carotene and information about its various lambda maxima by visiting the following link.

beta-Carotene, Lycopene can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using Vis.

Condition

Description

HPLC Method for Analysis of Lycopene, beta-Carotene on Lipak Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Lycopene, beta-Carotene.

Lycopene is a carotenoid hydrocarbon with C₄₀H₅₆ molecular formula. It is found primarily in tomatoes, watermelon, grapefruits, and red guavas. Gac has the highest concentration of lycopene at 2~6 per gram. Lycopene in non-toxic, but can be a rare allergen. You can find detailed UV spectra of Lycopene and information about its various lambda maxima by visiting the following link.

beta-Carotene, also written as β-Carotene, is a pigment with the chemical formula C₄₀H₅₆. It is found primarily in fungi, plants, and fruit. Especially high amounts of it are found in carrots, pumpkins, spinach, and sweet potatoes. In dietary contexts, it is used as a pro-vitamin, as it converts to retinol in the body. In fact, it is the worldwide primary dietary source of provitamin A and is the best known provitamin a carotenoid. You can find detailed UV spectra of beta-Carotene and information about its various lambda maxima by visiting the following link.

*Lycopene  and β – Carotene  Extraction from Tomato

Weigh 5.0 g of homogenized tomato sample into a centrifuge tube.

Add 10.0 mL of hexane/acetone (3:1, v/v).

Mix the sample using an ika ultra turrax tube drive until  or an equivalent tube homogenizer/mixer until the sample is thoroughly homogenized and extracted.

Centrifuge for 5–10 minutes to separate the solid matrix from the organic extract.

Carefully transfer the clear organic supernatant to a clean tube.

Protect the extract from direct light throughout the procedure.

If necessary, dilute the extract with the extraction solvent to obtain a suitable concentration for HPLC analysis.

Filter the final solution through a 0.45 µm PTFE filter before injection.

Inject 2 µL of the filtered extract into the HPLC system.

Lycopene, beta-Carotene can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) and ethanol. Detection is performed using Vis.

Condition

Description

*Lycopene and β – Carotene Extraction from Tomato
Weigh 5.0 g of homogenized tomato sample into a centrifuge tube.
Add 10.0 mL of hexane/acetone (3:1, v/v).
Mix the sample using an ika ultra turrax tube drive until or an equivalent tube homogenizer/mixer until the sample is thoroughly homogenized and extracted.
Centrifuge for 5–10 minutes to separate the solid matrix from the organic extract.
Carefully transfer the clear organic supernatant to a clean tube.
Protect the extract from direct light throughout the procedure.
If necessary, dilute the extract with the extraction solvent to obtain a suitable concentration for HPLC analysis.
Filter the final solution through a 0.45 µm PTFE filter before injection.
Inject 2 µL of the filtered extract into the HPLC system.

HPLC Method for beta-Carotene on Lipak by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of beta-Carotene

beta-Carotene, also written as β-Carotene, is a pigment with the chemical formula C₄₀H₅₆. It is found primarily in fungi, plants, and fruit. Especially high amounts of it are found in carrots, pumpkins, spinach, and sweet potatoes. In dietary contexts, it is used as a pro-vitamin, as it converts to retinol in the body. In fact, it is the worldwide primary dietary source of provitamin A and is the best known provitamin a carotenoid. You can find detailed UV spectra of beta-Carotene and information about its various lambda maxima by visiting the following link.

beta-Carotene is analyzed using a Lipak stationary phase column under isocratic conditions with a mobile phase composed of acetonitrile (MeCN) and ethanol. Detection is performed by visible (Vis) absorbance detection

Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze beta-Carotene check our HPLC Applications library

UV/Vis.: λmax: 275, 452, 478 nm

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in acetonitrile. This analysis was done with the mobile phase made of acetonitrile.