| CAS Number | 7235-40-7 |
|---|---|
| Molecular Formula | C40H56 |
| Molecular Weight | 536.889 |
| InChI Key | OENHQHLEOONYIE-JLTXGRSLSA-N |
| LogP | 13.5 |
| Synonyms |
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Applications:
HPLC Method for Analysis of beta-Carotene on Lipak Column
May 14, 2026
HPLC Method for beta-Carotene on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of beta-Carotene
beta-Carotene, also written as β-Carotene, is a pigment with the chemical formula C40H56. It is found primarily in fungi, plants, and fruit. Especially high amounts of it are found in carrots, pumpkins, spinach, and sweet potatoes. In dietary contexts, it is used as a pro-vitamin, as it converts to retinol in the body. In fact, it is the worldwide primary dietary source of provitamin A and is the best known provitamin a carotenoid. You can find detailed UV spectra of beta-Carotene and information about its various lambda maxima by visiting the following link.
beta-Carotene is analyzed using a Lipak stationary phase column under isocratic conditions with a mobile phase composed of acetonitrile (MeCN) and ethanol. Detection is performed by visible (Vis) absorbance detection
| Column | Lipak, 3.2 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN/EtOH – 70/30% |
| Buffer | None |
| Flow Rate | 0.5 ml/min |
| Detection | Vis 450 nm |
| Limit of Detection* | 30ppb |
| Class of Compounds | Pigment |
| Analyzing Compounds | beta-Carotene |
Application Column
Lipak
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Uv-Vis Spectrum of beta-Carotene
May 14, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze beta-Carotene check our HPLC Applications library
UV/Vis.: λmax: 275, 452, 478 nm
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The compound was dissolved in acetonitrile. This analysis was done with the mobile phase made of acetonitrile.
Separation of beta-Carotene on Newcrom R1 HPLC column
February 16, 2018
beta-Carotene can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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