HPLC Method for Analysis of beta-Carotene on Lipak Column

HPLC Method for beta-Carotene on Lipak by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of beta-Carotene

beta-Carotene, also written as β-Carotene, is a pigment with the chemical formula C40H56. It is found primarily in fungi, plants, and fruit. Especially high amounts of it are found in carrots, pumpkins, spinach, and sweet potatoes. In dietary contexts, it is used as a pro-vitamin, as it converts to retinol in the body. In fact, it is the worldwide primary dietary source of provitamin A and is the best known provitamin a carotenoid. You can find detailed UV spectra of beta-Carotene and information about its various lambda maxima by visiting the following link.

beta-Carotene is analyzed using a Lipak stationary phase column under isocratic conditions with a mobile phase composed of acetonitrile (MeCN) and ethanol. Detection is performed by visible (Vis) absorbance detection

ColumnLipak, 3.2 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN/EtOH – 70/30%
BufferNone
Flow Rate0.5 ml/min
DetectionVis 450 nm
Limit of Detection*30ppb
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Pigment
Analyzing Compoundsbeta-Carotene

Application Column

Lipak

Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
beta-Carotene
Application Detection:
Vis Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.