Alexidine

CAS Number22573-93-9
Molecular Formula C26H56N10
Molecular Weight508.8
InChI KeyLFVVNPBBFUSSHL-UHFFFAOYSA-N
LogP3.3
Synonyms

    Applications:

    HPLC MS Method for Analysis of  Alexidine on Primesep N Column

    March 8, 2024

    HPLC Method for Analysis of Alexidine on Primesep N Column by SIELC Technologies

    Separation type: Liquid Chromatography Reversed-phase

     HPLC MS Method for Analysis of  Alexidine on Primesep N Column
    HPLC Method for Analysis of Alexidine on Primesep N Column by SIELC Technologies

    Alexidine is a chemical compound that belongs to the class of bis-biguanide antiseptics. It is known for its antimicrobial properties and is used in various products for disinfection and antiseptic purposes. Alexidine has broad-spectrum antimicrobial activity, and it is commonly used in healthcare settings for its effectiveness against bacteria, fungi, and some viruses.

    Alexidine can be retained and analyzed on a Primesep N mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a ammonium formate as a buffer. This analysis method can be detected using LC MS.

    High Performance Liquid Chromatography (HPLC) Method for Analyses of Alexidine on Primesep N Column by SIELC Technologies

    Condition

    ColumnPrimesep N, 2.1 x 100 mm, 5 µm, 100 A
    Mobile PhaseMeCN/H2O – 85/15%
    BufferAmmonium Formate pH 3.0 – 10 mM
    Flow Rate0.2 ml/min
    DetectionSIM 255+, 509+

    Description

    Class of CompoundsBis-biguanide
    Analyzing CompoundsAlexidine

    Application Column

    Primesep N

    Column Diameter: 2.1 mm
    Column Length: 100 mm
    Particle Size: 5 µm
    Pore Size: 100 A

    Add to cart
    Application Analytes:
    Alexidine

    Application Detection:
    LC MS Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Method for Separation Alexidine and Polyhexanide on BIST B+ Column

    January 23, 2024

    High Performance Liquid Chromatography (HPLC) Method for Analysis of Polyhexanide PHMB, Alexidine on BIST B+ by SIELC Technologies

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

    HPLC Method for Separation Alexidine and Polyhexanide on BIST B+ Column
    HPLC Method for Analysis of Polyhexanide PHMB, Alexidine on on BIST B+ by SIELC Technologies

    Alexidine and polyhexanide are both antiseptic agents used for various applications, particularly in the field of medicine and healthcare.

    1. Alexidine:
      • Usage: Alexidine is a bisbiguanide antiseptic that is often used in oral care products, such as mouthwashes and dental formulations.
      • Antimicrobial Properties: It has broad-spectrum antimicrobial properties, effective against bacteria, fungi, and some viruses.
      • Application: Alexidine has been studied for its use in controlling oral biofilm and preventing dental plaque.
    2. Polyhexanide:
      • Usage: Polyhexanide is a polymeric biguanide antiseptic that is used in various medical applications, including wound care and disinfection.
      • Antimicrobial Properties: Similar to alexidine, polyhexanide has broad-spectrum antimicrobial properties and is effective against bacteria, fungi, and some viruses.
      • Application: Polyhexanide is commonly used as an antiseptic solution for wound irrigation and for the treatment of infected wounds. It can also be found in some contact lens solutions.

    Both alexidine and polyhexanide belong to the biguanide class of antiseptics, and they are known for their effectiveness against a wide range of microorganisms. The specific applications and formulations may vary based on the intended use, and these agents are often used in healthcare settings to prevent infections.

    Alexidine and polyhexanide can be retained, separated and analyzed on a BIST B+ mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a sulfuric acid as a buffer. This analysis method can be detected using UV at 200 nm.

    High Performance Liquid Chromatography (HPLC) Method for Analyses of Polyhexanide PHMB, Alexidine

    Condition

    ColumnBIST B+, 4.6 x 150 mm, 5 µm, 100 A
    Mobile Phase*Step Gradient
      A: MeCN – 75%, H2SO4 – 0.1% / 3 min
      B: MeCN – 0%, H2SO4 – 0.1% / 6 min
    Buffe H2SO4 -0.1%
    Flow Rate1.0 ml/min
    DetectionUV 200 nm
    Sample 1.  0.14 mg/ml
    2.  0.26 mg/ml
    LOD **8 ppb, 10 ppb
    Injection volume10 µl

    *The step gradient methods involve sudden changes in the solvent composition of the mobile phase, resulting in the baseline’s instability. It is necessary to account for this response to be able to interpret, integrate, and process results adequately. The blank sample (sample diluent) has to be analyzed before injecting the sample of interest. When all chromatograms are generated, it is necessary to subtract the blank chromatogram from the sample one before the integration step.

    **LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed

    Description

    Class of CompoundsBis-biguanide
    Analyzing CompoundsPolyhexanide PHMB, Alexidine

    Application Column

    BIST B+

    Column Diameter: 4.6 mm
    Column Length: 150 mm
    Particle Size: 5 µm
    Pore Size: 100 A

    Add to cart
    Application Analytes:
    Alexidine
    Polyhexanide PHMB

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

    HPLC Method for Analysis of Alexidine on BIST B+ Column

    January 16, 2024

    High Performance Liquid Chromatography (HPLC) Method for Analysis of Alexidine on BIST B+ by SIELC Technologies

    Separation type: Bridge Ion Separation Technology, or BIST™ by SIELC Technologies

    HPLC Method for Analysis of Alexidine on on BIST B+ by SIELC Technologies

    Alexidine is a chemical compound that belongs to the class of bis-biguanide antiseptics. It is known for its antimicrobial properties and is used in various products for disinfection and antiseptic purposes. Alexidine has broad-spectrum antimicrobial activity, and it is commonly used in healthcare settings for its effectiveness against bacteria, fungi, and some viruses.

    Topical Antiseptics: In some formulations, alexidine is used as a topical antiseptic to disinfect skin and mucous membranes.

    Oral Care Products: It is found in some mouthwashes and oral care products due to its antimicrobial properties.

    Contact Lens Solutions: Alexidine is sometimes used in contact lens solutions to prevent microbial contamination.

    Wound Care Products: It may be included in certain wound care products for its ability to prevent infection.

    Alexidine can be retained and analyzed on a BIST B+ mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a sulfuric acid as a buffer. This analysis method can be detected using UV at 200 nm.

    High Performance Liquid Chromatography (HPLC) Method for Analyses of Alexidine

    Condition

    ColumnBIST B+, 4.6 x 50 mm, 5 µm, 100 A
    Mobile PhaseMeCN – 82%
    Buffe H2SO4 -0.1%
    Flow Rate1.0 ml/min
    DetectionUV 200 nm, UV λmax: 237 nm
    Sample0.4 mg/ml in MeCN/H2O – 82/18%
    LOD8 ppb
    Injection volume2 µl

    Description

    Class of CompoundsBis-biguanide
    Analyzing CompoundsAlexidine

    Application Column

    BIST B+

    Column Diameter: 4.6 mm
    Column Length: 50 mm
    Particle Size: 5 µm
    Pore Size: 100 A

    Add to cart
    Application Analytes:
    Alexidine

    Application Detection:
    UV Detection
    SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.