High Performance Liquid Chromatography (HPLC) Method for Analysis of Alexidine on BIST B+ by SIELC Technologies
Alexidine is a chemical compound that belongs to the class of bis-biguanide antiseptics and has the chemical formula C26H56N10. It is known for its antimicrobial properties and is used in various products for disinfection and antiseptic purposes. Alexidine has broad-spectrum antimicrobial activity, and it is commonly used in healthcare settings for its effectiveness against bacteria, fungi, and some viruses. In some formulations, alexidine is used as a topical antiseptic to disinfect skin and mucous membranes. It is found in some mouthwashes, oral care products, contact lens solutions, and more It may be included in certain wound care products for its ability to prevent infection. You can find detailed UV spectra of Alexidine and information about its various lambda maxima by visiting the following link.
Alexidine can be retained and analyzed on a BIST B+ mixed-mode stationary phase column using an analytical method with a simple mobile phase of water, Acetonitrile (MeCN) , and a sulfuric acid as a buffer. This analysis method can be detected using UV at 200 nm.
Condition
| Column | BIST B+, 4.6 x 50 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 82% |
| Buffe | H2SO4 -0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 200 nm, UV λmax: 237 nm |
| Sample | 0.4 mg/ml in MeCN/H2O – 82/18% |
| LOD | 8 ppb |
| Injection volume | 2 µl |
Description
| Class of Compounds | Bis-biguanide |
| Analyzing Compounds | Alexidine |
Application Column
BIST B+
Column Diameter: 4.6 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
