Glutamine

Glutamine

CAS Number6899-04-3
Molecular FormulaC5H10N2O3
Molecular Weight146.146
InChI KeyZDXPYRJPNDTMRX-UHFFFAOYSA-N
LogP-2.95
Synonyms
  • DL-Glutamine
  • Glutamine
  • 6899-04-3
  • Glutamine
  • EINECS 230-006-0
  • (+-)-Glutamine
  • gamma-Glutamine
  • Glutamine DL-form
  • NSC 97925
  • 2,5-diamino-5-oxopentanoic acid
  • 2-amino-4-carbamoylbutanoic acid
  • Glutamin
  • Glutaminsaeure-5-amid
  • Hgln
  • glutamic acid gamma-amide
  • 585-21-7

Applications:

UV-Vis Spectrum of Glutamine

July 9, 2024

Access the UV-Vis Spectrum SIELC Library

UV-Vis Spectrum of [compounds]. Absorption starts high at 200 nm but quickly falls off and bottoms out around 230 nm.

If you are looking for optimized HPLC method to analyze Glutamine check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

Application Analytes:
Glutamine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of Amino Acids in Supplements Capsules

April 10, 2005

Glutamine, valine, isoleucine, and leucine are baseline resolved in a dietary supplement with a mobile phase of acetonitrile, water, and sulfuric acid with ultraviolet (UV) detection at 210 nm on a Primesep 100 column. Increasing the sulfuric acid concentration allows elution of arginine. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), and sulfuric acid with ultraviolet (UV) detection at 210 nm.

Condition
Column Primesep 100, 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer H2SO4
Flow Rate 1.0 ml/min
Detection UV, 210 nm

 

Description
Class of Compounds
 Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Arginine, Glutamine,  L-Valine (Val/V),  L-Isoleucine (Ile/I), L-Leucine (Leu/L)

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Arginine
Glutamine
Isoleucine
Leucine
Valine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.