Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Copper
Copper has been known to man since ancient times. In today’s modern world, copper can be found in electronic equipment, complex biological processes, and as an antimicrobial agent due to its biostatic properties.
Copper (II) ion can be detected in the low UV regime. Using a Primesep 100 reverse-phase column and a mobile phase consisting of water and acetonitrile (MeCN) with an perchloric acid (HClO4) buffer, copper (II) can be retained, measured, and analyzed. Modifying the relative concentrations of MeCN, water, and HClO4 can increase the retention time. This analysis method can be UV detected at 200 nm.
||Primesep 100, 4.6x100 mm, 5 µm, 100A
||UV, 200 nm
|Class of Compounds