A unique approach for analysis of copper ions in a high salt content matrix was developed using EDTA as a visualization agent. Since copper ions can form colored complexes with EDTA, this property can be used for selective analysis of copper in the mixture with other cations, which do not form a colored complex with EDTA. Copper ion can be monitored by UV while other metals pass through the column undetected by UV. This method can be used to analyze copper ion at parts per million concentrations.
| Column | Primesep SB, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmAc, EDTA |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 310 nm |
| Class of Compounds |
Ions |
| Analyzing Compounds | Copper |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCopper Sulfate
