HPLC UV Analysis of Copper Ions in Salt Mixture

A unique approach for analysis of copper ions in a high salt content matrix was developed using EDTA as a visualization agent. Since copper ions can form colored complexes with EDTA, this property can be used for selective analysis of copper in the mixture with other cations, which do not form a colored complex with EDTA. Copper ion can be monitored by UV while other metals pass through the column undetected by UV. This method can be used to analyze copper ion at parts per million concentrations.


Column Primesep SB, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmAc, EDTA
Flow Rate 1.0 ml/min
Detection UV, 310 nm


Class of Compounds Ions
Analyzing Compounds Copper
Application Analytes:
Copper Sulfate