18:0 Lyso PG

CAS Number326495-23-2
Molecular FormulaC24H48O9PNa
Molecular Weight534.596
Synonyms
  • 1-stearoyl-2-hydroxy-sn-glycero-3-phosphoglycerol (sodium salt)
  • LPG(18:0/0:0)
  • Lysophosphatidylglycerol 18:0 (sodium salt)
  • 1-octadecanoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt)

Applications:

ELSD-HPLC Method for  Analysis of Phospholipids on Lipak Colulm

November 22, 2024

ELSD-HPLC Method for Phospholipids on Lipak by SIELC Technologies

HPLC Method for Analysis of Phospholipids on Lipak Column by SIELC Technologies

Phospholipids are vital components of cell membranes, playing key roles in cell signaling, energy storage, and protective functions. They are essential for maintaining cellular structure and function, making them crucial in biological processes and industrial applications such as drug delivery and cosmetic formulations.

Over time, phospholipids can degrade into various biologically active products, including lysophospholipids and fatty acids, which are involved in cellular signaling and inflammation. Monitoring both phospholipids and their degradation products provides valuable insights into metabolic health, lipid metabolism, and the stability of lipid-based formulations.

Our high-resolution chromatograms ensure accurate and reliable analysis of both phospholipids and their degradation products, supporting applications in clinical diagnostics, pharmaceuticals, and food quality control.

DEPC, DSPG, 18:0 Lyso PG, Cholesterol, Dipalmitoylphosphatidylcholine (DPPC) can be retained, and analyzed using a Lipak mixed-mode stationary phase column. The analysis utilizes an gradient method with a mobile phase consisting of water, methanol (MeOH), ammonium formate and formic acid as a buffer. Detection is achieved using ELSD

ColumnLipak, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseGradient MeOH –90-100% 20 min, 10 min hold
BufferAmFm– 10 mM, FA – 0.05%
Flow Rate1.0 ml/min
DetectionELSD, the nebulizer and evaporator temperatures 40°C,
with a gas flow rate of 1.6 Standard Liters per Minute (SLM)

Class of Compounds
Phospholipids
Analyzing CompoundsDEPC, DSPG, 18:0 Lyso PG, Cholesterol, Dipalmitoylphosphatidylcholine (DPPC)

Application Column

Lipak

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
18:0 Lyso PG
Cholesterol
DEPC
DSPG
Dipalmitoylphosphatidylcholine (DPPC)
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.