| CAS Number | 57-88-5 |
|---|---|
| Molecular Formula | C27H46O |
| Molecular Weight | 386.664 |
| InChI Key | HVYWMOMLDIMFJA-DPAQBDIFSA-N |
| LogP | 8.45 |
| Synonyms |
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Applications:
Uv-Vis Spectrum of Cholesterol
April 2, 2026
Access the UV-Vis Spectrum SIELC Library
If you are looking for optimized HPLC method to analyze Cholesterol check our HPLC Applications library
For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.
HPLC ELSD Method for Analysis of Cholesterol on Lipak Column
October 3, 2025
HPLC Method for Cholesterol on Lipak by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Cholesterol
Cholesterol is a compound that is essential for cell function with the molecular formula C27H46O. High levels of cholesterol can lead to heart disease and stroke due to potential accumulation in the arteries. It is caused through a variety of causes includinggenetics, an unhealthy diet, lack of exercise, smoking, and certain medical conditions.
Cholesterol can be retained and analyzed using the Lipak stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and methanol (MeOH) with a formic acid buffer. Detection is performed using ELSD.
*LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.
| Column | Lipak, 3.2 x 150 mm, 3 µm, 100 A, dual ended |
| Mobile Phase | MeOH – 99.5% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 0.5 ml/min |
| Detection | ELSD |
| Class of Compounds | Cholestanoid |
| Analyzing Compounds | Cholesterol |
Application Column
Lipak
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 3 µm
Pore Size: 100 A
Column options: dual ended
Separation of Cholesterol on Newcrom R1 HPLC column
February 16, 2018
Cholesterol can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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