EDTA (Ethylenediaminetetraacetic Acid)

EDTA (Ethylenediaminetetraacetic Acid) structural formula

CAS Number60-00-4
Molecular FormulaC10H16N2O8
Molecular Weight292.244
InChI KeyKCXVZYZYPLLWCC-UHFFFAOYSA-N
LogP-1.74
Synonyms
  • Ethylenediaminetetraacetic acid
  • 2,2',2'',2'''-(Ethane-1,2-diyldinitrilo)tetraacetic acid
  • 60-00-4
  • Edetic acid
  • Glycine, N,N'-1,2-ethanediylbis[N-(carboxymethyl)-
  • EDTA
  • 1,2-Bis (N,N-dicarboxymethylamino)-ethane
  • 3,6-Diazaoctanedioic acid, 3,6-bis(carboxymethyl)-
  • Acetic acid, (ethylenedinitrilo)tetra-
  • Acetic acid, 2,2',2'',2'''-(1,2-ethanediyldinitrilo)tetrakis-
  • Acide edetique
  • acido edetico
  • Acroma DH 700
  • AETHYLENDIAMIN-TETRAESSIGSAEURE
  • Celon A
  • Celon ATH
  • Cheelox
  • Chemcolox 340
  • Clewat TAA
  • Clewat TTA
  • Complexon II
  • Dissolvine E
  • Dissolvine Z
  • DOHTITE 4H, EDTA ACID
  • Edathamil
  • EDETA B
  • Edetinsaure
  • EDTA ACID FORM
  • Endrate
  • ETHYLENE DINITRILO TETRAACETIC ACID
  • Ethylenediamine Tetra-Acetic Acid
  • Ethylenediamine-N,N,N',N'-tetraacetic acid
  • Ethylenediaminetetracetic acid
  • ETHYLENEDINITRILOTETRAACETIC ACID
  • Ethylene-N,N'-biscarboxymethyl-N,N'-diglycine
  • Gluma Cleanser
  • GLYCINE, N,N'-1,2-ETHANEDIYLBIS(N-(CARBOXYMETHYL))
  • Glycine,N,N'-1,2-ethanediylbis[N-(carboxymethyl)-,
  • Havidote
  • Metaquest A
  • N,N'-1,2-Ethanediylbis[N-(carboxymethyl)glycine]
  • N,N'-1,2-Ethanediyl-bis-N-(carboxymethyl)glycine
  • Nervanaid B acid
  • NSC 97243
  • NSC 97404
  • Nullapon B acid
  • Nullapon BF acid
  • Perma Kleer 50 acid
  • Quastal Special
  • Sequestrene AA
  • Sequestrene K 4
  • Sequestric acid
  • Sequestrol
  • Techrun DO
  • Titriplex
  • Titriplex II
  • Trilon BS
  • Trilon BW
  • Versene
  • Zonon AO
  • BRN 1716295
  • Caswell No. 438
  • Cheelox BF acid
  • EDTA acid
  • EINECS 200-449-4
  • EPA Pesticide Chemical Code 039101
  • Ethylenebisiminodiacetic acid
  • (Ethylenedinitrilo)tetraacetic acid
  • Hamp-ene acid
  • Komplexon II
  • Tetrine acid
  • Universne acid
  • Versene acid
  • Vinkeil 100
  • Warkeelate acid
  • Acide ethylenediaminetetracetique
  • Acidum edeticum
  • Kyselina ethylendiamintetraoctova
  • UNII-9G34HU7RV0
  • Ethylenebis(iminodiacetic acid)
  • 13440-78-3
  • 161122-33-4
  • 20539-27-9
  • 26627-46-3
  • 30485-87-1
  • 30485-88-2
  • 30485-90-6
  • 32757-10-1
  • 402925-67-1
  • 675141-16-9
  • 94108-75-5

Applications:

HPLC Method for Analysis of EDTA and Maleic Acid on Newcrom B Column

July 26, 2022

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of EDTA and Maleic Acid on Newcrom B Column

EDTA Standards Solution A:
For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride Solution B:
The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric EDTA complex analysis:
To make a sample for analysis mix 100 µL Solution A (or unknown sample) with 100 µL Solution B and 800 µL of water.  Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA and Maleic Acid

Condition

Column Newcrom B, 4.6×150 mm,  100A
Mobile Phase H2O – 99.98%
Buffer H2SO4 – 0.02%
Flow Rate 1.0 ml/min
Detection UV 260 nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds EDTA, Maleic Acid

New more reliable method for EDTA analysis is available

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
EDTA (Ethylenediaminetetraacetic Acid)
Iron(III)
Maleic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method For Analysis Of EDTA and Maleic Acid

April 28, 2022

Separation type: Liquid Chromatography Mixed-mode

 


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Ethylenediaminetetraacetic acid (EDTA) is a very common chelating agent, used particularly for collecting Iron and Calcium ions. It has a wide range of applications, including in the textile industry, paper industry, pharmaceutical industry, and in cosmetics, where it is used to capture unwanted metal ions in solution. Maleic acid, a dicarboxylic acid, has a few interesting applications, such in medicine to form salts with drugs to increase their stability and as a precursor for the production of glyoxylic acid, which is used in cosmetics. An Iron (III)-EDTA complex and Maleic acid can both be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of (mostly) water, Acetonitrile (MeCN), and Perchloric acid (HClO4). This analytical method can be UV detected at 220 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA and  Maleic Acid

Condition

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase MeCN – 2%
Buffer HClO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 220nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds EDTA, Maleic Acid

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
EDTA (Ethylenediaminetetraacetic Acid)
Iron(III)
Maleic Acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of Nitrilotriacetic acid (NTA) and Ethylenediaminetetraacetic acid (EDTA) on Newcrom BH Column

September 2, 2021


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NTA Standards Solution A: For the preparation of the NTA standard solution, 5 mg of NTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water with sonication. The NTA stock solution (1 mg/mL) should be stored in a cold, dark place and can be used for upto a week to prepare standards of required concentration.

Nitrilotriacetic acid (NTA) and Ethylenediaminetetraacetic acid (EDTA) are two common chelating agents used often in laboratory and medical testing. They are often used to bind to, identify, and analyze metal ions. Below is a method we developed to retain and measure Fe (III) using NTA and EDTA complexes on a Newcrom BH mixed-mode column, with a mobile phase of acetonitrile (MeCN), water, and a buffer of either sulfuric acid (H2SO4) or formic acid:

EDTA Standards Solution B: For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL)  should be stored in a cold dark  place and can be used for a week to prepare standards of required concentration.
NTA Standard – Solution A: For the preparation of the NTA standard solution, 5 mg of NTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water with sonication. The NTA stock solution (1 mg/mL) should be stored in a cold, dark place and can be used for upto a week to prepare standards of required concentration.

EDTA Standard – Solution B: For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filter the EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride – Solution C: The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric NTA and EDTA complex analysis: To make a sample for analysis, mix 100 µL each Solution A and Solution B (or unknown sample) with 100 µL Solution C and 700 µL of water. Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

 

Iron(III) chloride Solution C: The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric NTA and EDTA  complexes analysis: To make a sample for analysis mix 100 µL each Solution A and Solution B (or unknown sample) with 100 µL Solution C and 700 µL of water. Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

 

 

 

Condition

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase MeCN/H2O – 2/98%
Buffer H2SO4 or Formic Acid
Flow Rate 1.0 ml/min
Detection UV 275nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds EDTA, NTA

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
EDTA (Ethylenediaminetetraacetic Acid)
Nitrilotriacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of EDTA on Newcrom BH Column

June 17, 2021


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EDTA Standards Solution A:
For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride Solution B:
The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric EDTA complex analysis:
To make a sample for analysis mix 100 µL Solution A (or unknown sample) with 100 µL Solution B and 800 µL of water.  Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

Condition

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase MeCN/H2O – 2/98%
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 260nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds EDTA

 

New mo

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
EDTA (Ethylenediaminetetraacetic Acid)
Iron(III)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Separation of EDTA, Oxymetazoline and Benzyl Alcohol in Nasal Spray

September 4, 2020

Separation type: Liquid Chromatography Mixed-mode




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High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA, Oxymetazoline and Benzyl Alcohol



Benzyl alcohol is found in over-the-counter medications, topical creams, lotions, shampoos, and facial cleansers as an antibacterial, preservative, and/or fungicide. Benzyl alcohol is a colorless liquid with a mild pleasant aromatic scent. Benzyl alcohol is found in many essential oils including jasmine, hyacinth, neroli, rose, and ylang-ylang. Oxymetazoline is a decongestant that shrinks blood vessels in the nasal passages. All active compounds of nasal spray can be separated in HPLC on a Newcrom BH mixed-mode column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 260nm.
 


Condition

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase Gradient MeCN – 2- 70%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 260nm

Description

Class of Compounds Acid, Hydrophilic, Preservatives
Analyzing Compounds EDTA, Oxymetazoline, Benzyl Alcohol

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Benzyl alcohol
EDTA (Ethylenediaminetetraacetic Acid)
Oxymetazoline
Oxymetazoline hydrochloride
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of EDTA, Phenoxyethanol and Chlorphenesin in Eye Makeup Remover on Newcrom BH Column

August 25, 2020


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Condition

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase Gradient MeCN – 2- 70%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 260nm

Description

Class of Compounds Acid, Hydrophilic, Preservatives
Analyzing Compounds EDTA, Chlorphenesin, Phenoxyethanol

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
2-Phenoxyethanol
Chlorphenesin
EDTA (Ethylenediaminetetraacetic Acid)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of EDTA, Phenoxyethanol and Methylparaben in Facial Cleanser on Newcrom BH

August 25, 2020


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Condition

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase Gradient MeCN – 2- 70%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 260nm

Description

Class of Compounds Acid, Hydrophilic, Preservatives
Analyzing Compounds EDTA, Methylparaben, Phenoxyethanol

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
2-Phenoxyethanol
EDTA (Ethylenediaminetetraacetic Acid)
Methylparaben
Methylparaben sodium
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Determination of EDTA on Newcrom B Column

April 22, 2020


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EDTA Standards Solution A:
For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride Solution B:
The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric EDTA complex analysis:
To make a sample for analysis mix 100 µL Solution A (or unknown sample) with 100 µL Solution B and 800 µL of water.  Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

Ethylenediaminetetraacetic acid (EDTA) is a very common chelating agent, used particularly for collecting Iron and Calcium ions. It has a wide range of applications, including in the textile industry, paper industry, pharmaceutical industry, and in cosmetics, where it is used to capture unwanted metal ions in solution. An Iron (III)-EDTA complex can be retained and analyzed on a mixed-mode Newcrom B column with a mobile phase consisting of (mostly) water, Acetonitrile (MeCN), and Sulfuric acid (H2SO4). This analytical method can be UV detected at 260 nm with high resolution and peak symmetry.

ColumnNewcrom B, 4.6×150 mm,  100A
Mobile PhaseH2O – 99.98%
BufferH2SO4 – 0.02%
Flow Rate1.0 ml/min
DetectionUV 260nm
Class of CompoundsAcid, Hydrophilic
Analyzing CompoundsEDTA

New more reliable method for EDTA analysis is available

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
EDTA (Ethylenediaminetetraacetic Acid)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Retention of EDTA in Sulfated CD

November 21, 2010


EDTA, or Ethylenediaminetetraacetic acid, is widely used in textile, pulp/paper, food and pharmaceutical industries. It has application as chelating agent and preservative. EDTA molecule is very hydrophilic and contains two basic groups and four carboxylic groups. It has tendency to bind to metals, making analysis of EDTA by HPLC very challenging. EDTA is analyzed in the presence of copper sulfate as visualization agent on a Primesep SB column. The method can be used for EDTA determination in various mixtures and compositions using UV detector.

Condition

Column Primesep  SB , 4.6×250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer MeSO3H with CuSO4
Flow Rate 1.0 ml/min
Detection UV 300 nm

 

Description

Class of Compounds
Acid
Analyzing Compounds EDTA, or Ethylenediaminetetraacetic acid

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
EDTA (Ethylenediaminetetraacetic Acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HILIC Separation of Common Preservatives – Citric Acid, Ascorbic Acid and EDTA

July 16, 2009

Citric acid, ascorbic acid, and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.

Condition

Column Obelisc N , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Acid, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Citric acid, ascorbic acid and EDTA

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Ascorbic Acid
EDTA (Ethylenediaminetetraacetic Acid)
Vitamin C

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Application for Analysis of EDTA

December 6, 2007

New more reliable method for EDTA analysis is available
 

EDTA (Ethylenediaminetetraacetic acid) is amino acid based compound which is widely used in industrial cleaners, detergents, photography, agrochemicals, textile, and food industries. EDTA serves as preservative in packaged food, vitamins and pharmaceutical formulations. It is used in organic and analytical laboratories as scavenger of metals, buffer solution, complexometric titration, masking agent for metal determination, etc.
EDTA is very polar compound with strong chelating properties. Low UV activity and strong binding to metal ions makes HPLC analysis of EDTA very difficult. Reproducible method for determination of wide range on concentration of EDTA in various formulations is developed using Primesep D anion-exchange mixed mode column. Copper sulfate is used for visualization purposes to increase sensitivity of the method. EDTA forms UV active complex with some metal ions. This method can be used for highly accurate quantitation of EDTA in different mixtures and composition. Method demonstrates controllable retention and perfect peak shape. Equilibration of the column prior to analysis requires special attention.

If multiple injections produce different retention times, your column might not be equilibrated properly. Primesep D columns have a large ionic capacity towards anions. The shipping solvent for the Primesep D columns is ACN/water/TFA. The end point of gradient has only 4 mmol of sulfate ions. It takes over 5 hours to equilibrate a column with such low concentration. Before running your experiments you need to wash your column with 20% ACN and 0.2% of sulfuric acid for 1 hour. After you replace previous buffer/additive, you can set up your experiment. If you don’t change ionic modifier in your mobile phase the equilibration time is equivalent of  4-5 column volumes.

Condition

Column Primesep  D , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4 with CuSO4
Flow Rate 1.0 ml/min
Detection UV 310 nm

 

Description

Class of Compounds
Acid
Analyzing Compounds EDTA, or Ethylenediaminetetraacetic acid

New more reliable method for EDTA analysis is available

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
EDTA (Ethylenediaminetetraacetic Acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.