Iron(III)

HPLC Method for Analysis of Iron(III) on Primesep NC by SIELC Technologies

Separation type: Hydrophilic interaction liquid chromatography (HILIC)

The analysis of Fe3+ involves various analytical methods such as colorimetric methods, atomic absorption spectroscopy (AAS), inductively coupled plasma optical emission spectrometry (ICP-OES), and high-performance liquid chromatography (HPLC). Within the realm of HPLC, specific techniques like ion chromatography or chelation chromatography are often employed to separate Fe3+ ions. Post-separation, UV-visible detectors or post-column derivatization with appropriate reagents can be used for detection and quantification of Fe3+ ions.

To overcome these complexities, SIELC has developed a novel, selective methodology, offering a straightforward and reliable approach for Fe(III) quantitation in various liquid samples. This method revolves around the formation of a complex with EDTA, which exhibits strong UV light absorption with peak maxima at 260 nm

       EDTA Standards Solution A

For the preparation of the Disodium Dihydrogen Ethylenediaminetetraacetate Dihydrate (EDTA disodium salt) standard solution, 20 mg of EDTA was accurately weighed and  transferred into a 10 mL volumetric flask and dissolved in water with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (2000 ppm)  should be stored in a cold dark  place and can be used for a week to prepare standards of required concentration.
Iron(III) chloride Solution B
The standard stock solution of Iron(III) chloride (100 ppm Fe3+) was prepared in water.  48 mg of Iron(III) chloride hexahydrate was accurately weighed and  transferred into a 100 mL volumetric flask and dissolved in water and sonicated if needed.
General procedure for Iron(III) – EDTA complex analysis
Mix 100 µL Solution A, 200 µL Solution B, and 700 µL of water. Place this mixture in a plastic HPLC vial for analysis.

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Iron(III)

Condition

Description

Separation type: Liquid Chromatography Mixed-mode

EDTA Standards Solution A:
For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride Solution B:
The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric EDTA complex analysis:
To make a sample for analysis mix 100 µL Solution A (or unknown sample) with 100 µL Solution B and 800 µL of water.  Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA and Maleic Acid

New more reliable method for EDTA analysis is available

Separation type: Liquid Chromatography Mixed-mode

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Ethylenediaminetetraacetic acid (EDTA) is a very common chelating agent, used particularly for collecting Iron and Calcium ions. It has a wide range of applications, including in the textile industry, paper industry, pharmaceutical industry, and in cosmetics, where it is used to capture unwanted metal ions in solution. Maleic acid, a dicarboxylic acid, has a few interesting applications, such in medicine to form salts with drugs to increase their stability and as a precursor for the production of glyoxylic acid, which is used in cosmetics. An Iron (III)-EDTA complex and Maleic acid can both be retained, separated, and analyzed on a mixed-mode Newcrom BH column with a mobile phase consisting of (mostly) water, Acetonitrile (MeCN), and Perchloric acid (HClO4). This analytical method can be UV detected at 220 nm with high resolution and peak symmetry.

High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA and  Maleic Acid

View on hplc.cloud

EDTA Standards Solution A:
For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride Solution B:
The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric EDTA complex analysis:
To make a sample for analysis mix 100 µL Solution A (or unknown sample) with 100 µL Solution B and 800 µL of water.  Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

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