HPLC Separation of EDTA, Oxymetazoline and Benzyl Alcohol in Nasal Spray

Separation type: Liquid Chromatography Mixed-mode

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High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA, Oxymetazoline and Benzyl Alcohol

Benzyl alcohol is found in over-the-counter medications, topical creams, lotions, shampoos, and facial cleansers as an antibacterial, preservative, and/or fungicide. Benzyl alcohol is a colorless liquid with a mild pleasant aromatic scent. Benzyl alcohol is found in many essential oils including jasmine, hyacinth, neroli, rose, and ylang-ylang. Oxymetazoline is a decongestant that shrinks blood vessels in the nasal passages. All active compounds of nasal spray can be separated in HPLC on a Newcrom BH mixed-mode column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 260nm.  


Column Newcrom BH, 4.6x150 mm,  100A
Mobile Phase Gradient MeCN - 2- 70%, 10 min
Buffer H2SO4 - 0.1%
Flow Rate 1.0 ml/min
Detection UV 260nm


Class of Compounds Acid, Hydrophilic, Preservatives
Analyzing Compounds EDTA, Oxymetazoline, Benzyl Alcohol
Application Analytes:
Benzyl alcohol
EDTA (Ethylenediaminetetraacetic Acid)
Oxymetazoline hydrochloride