Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA, Oxymetazoline and Benzyl Alcohol
Benzyl alcohol is found in over-the-counter medications, topical creams, lotions, shampoos, and facial cleansers as an antibacterial, preservative, and/or fungicide. Benzyl alcohol is a colorless liquid with a mild pleasant aromatic scent. Benzyl alcohol is found in many essential oils including jasmine, hyacinth, neroli, rose, and ylang-ylang. Oxymetazoline is a decongestant that shrinks blood vessels in the nasal passages. All active compounds of nasal spray can be separated in HPLC on a Newcrom BH mixed-mode column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 260nm.
| Column | Newcrom BH, 4.6×150 mm, 100A |
| Mobile Phase | Gradient MeCN – 2- 70%, 10 min |
| Buffer | H2SO4 – 0.1% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 260nm |
| Class of Compounds | Acid, Hydrophilic, Preservatives |
| Analyzing Compounds | EDTA, Oxymetazoline, Benzyl Alcohol |
Application Column
Newcrom BH
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsEDTA (Ethylenediaminetetraacetic Acid)
Oxymetazoline
Oxymetazoline hydrochloride
