CAS Number | 2315-02-8 |
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Molecular Formula | C16H25ClN2O |
Molecular Weight | 296.840 |
InChI Key | BEEDODBODQVSIM-UHFFFAOYSA-N |
LogP | 3.79 |
Synonyms |
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Applications:
HPLC Separation of EDTA, Oxymetazoline and Benzyl Alcohol in Nasal Spray
September 4, 2020
HPLC Method for EDTA (Ethylenediaminetetraacetic Acid), Oxymetazoline, Benzyl alcohol, Oxymetazoline hydrochloride on Newcrom BH by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA, Oxymetazoline and Benzyl Alcohol
Benzyl alcohol is found in over-the-counter medications, topical creams, lotions, shampoos, and facial cleansers as an antibacterial, preservative, and/or fungicide. Benzyl alcohol is a colorless liquid with a mild pleasant aromatic scent. Benzyl alcohol is found in many essential oils including jasmine, hyacinth, neroli, rose, and ylang-ylang. Oxymetazoline is a decongestant that shrinks blood vessels in the nasal passages. All active compounds of nasal spray can be separated in HPLC on a Newcrom BH mixed-mode column. The analytical method uses acetonitrile (ACN) gradient and water with sulfuric acid (H2SO4) as buffer and UV detected at 260nm.
Column | Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | Gradient MeCN – 2- 70%, 10 min |
Buffer | H2SO4 – 0.1% |
Flow Rate | 1.0 ml/min |
Detection | UV 260nm |
Class of Compounds | Acid, Hydrophilic, Preservatives |
Analyzing Compounds | EDTA (Ethylenediaminetetraacetic Acid), Oxymetazoline, Benzyl alcohol, Oxymetazoline hydrochloride |
Application Column
Newcrom BH
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
EDTA (Ethylenediaminetetraacetic Acid)
Oxymetazoline
Oxymetazoline hydrochloride
Separation of Oxymetazoline hydrochloride on Newcrom R1 HPLC column
May 16, 2018
Oxymetazoline hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
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