Nitrilotriacetic acid

Nitrilotriacetic acid structural formula

CAS Number139-13-9
Molecular FormulaC6H9NO6
Molecular Weight191.140
InChI KeyMGFYIUFZLHCRTH-UHFFFAOYSA-N
LogP-2.04
Synonyms
  • Nitrilotriacetic acid
  • 2,2',2''-Nitrilotriacetic acid
  • Glycine, N,N-bis(carboxymethyl)-
  • 139-13-9
  • NTA
  • 4-04-00-02441
  • Glycine, N,N-bis(carboxymethyl)-
  • Acetic acid, nitrilotri-
  • Acide nitrilotriacetique
  • acido nitrilotriacetico
  • Aminotriacetic acid
  • Chelest NT
  • Complexon I
  • Dissolvine A
  • Hampshire NTA acid
  • N,N-Bis(carboxymethyl)glycine
  • Nitrilo-2,2',2-triacetic acid
  • Nitrilo-2,2',2''-triacetic acid
  • Nitrilo-N,N,N-triacetic acid
  • NITRILOTRIESSIGSAEURE
  • Nitrilotriessigsaure
  • Nitrilotris(methylenecarboxylic acid)
  • NSC 2121
  • NTA N,N-Bis(carboxymethyl)glycine
  • Titriplex I
  • Tricollamic acid
  • Triglycollamic acid
  • Tris(hydroxycarbonylmethyl)amine
  • Versene NTA acid
  • ?,?',?''-Trimethylaminetricarboxylic acid
  • BRN 1710776
  • EINECS 205-355-7
  • Komplexon I
  • NCI-C02766
  • Nitriloacetate
  • Nitrilotriacetate
  • Tri(carboxymethyl)amine
  • Triglycine
  • alpha,alpha',alpha''-Trimethylaminetricarboxylic acid
  • Kyselina nitrilotrioctova
  • UNII-KA90006V9D
  • 26627-44-1
  • 26627-45-2
  • 80751-51-5

Applications:

HPLC Determination of Nitrilotriacetic acid (NTA) and Ethylenediaminetetraacetic acid (EDTA) on Newcrom BH Column

September 2, 2021


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NTA Standards Solution A: For the preparation of the NTA standard solution, 5 mg of NTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water with sonication. The NTA stock solution (1 mg/mL) should be stored in a cold, dark place and can be used for upto a week to prepare standards of required concentration.

Nitrilotriacetic acid (NTA) and Ethylenediaminetetraacetic acid (EDTA) are two common chelating agents used often in laboratory and medical testing. They are often used to bind to, identify, and analyze metal ions. Below is a method we developed to retain and measure Fe (III) using NTA and EDTA complexes on a Newcrom BH mixed-mode column, with a mobile phase of acetonitrile (MeCN), water, and a buffer of either sulfuric acid (H2SO4) or formic acid:

EDTA Standards Solution B: For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filtered The EDTA stock solution (1.0 mg/mL)  should be stored in a cold dark  place and can be used for a week to prepare standards of required concentration.
NTA Standard – Solution A: For the preparation of the NTA standard solution, 5 mg of NTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water with sonication. The NTA stock solution (1 mg/mL) should be stored in a cold, dark place and can be used for upto a week to prepare standards of required concentration.

EDTA Standard – Solution B: For the preparation of the EDTA standard solution, 5 mg of EDTA was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in 0.001N NaOH water solution with sonication or magnetic stirrer mixing. Filter the EDTA stock solution (1.0 mg/mL) should be stored in a cold dark place and can be used for a week to prepare standards of required concentration.

Iron(III) chloride – Solution C: The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric NTA and EDTA complex analysis: To make a sample for analysis, mix 100 µL each Solution A and Solution B (or unknown sample) with 100 µL Solution C and 700 µL of water. Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

 

Iron(III) chloride Solution C: The standard stock solution of Iron(III) chloride (10 mg/ml) was prepared in water. 50 mg of FeCl3 was accurately weighed and transferred into a 5 mL volumetric flask and dissolved in water, with sonication if needed.

General procedure for Ferric NTA and EDTA  complexes analysis: To make a sample for analysis mix 100 µL each Solution A and Solution B (or unknown sample) with 100 µL Solution C and 700 µL of water. Place this mixture in a plastic HPLC vial for analysis. Setup instrument and column according to the method provided.

 

 

 

Condition

Column Newcrom BH, 4.6×150 mm,  100A
Mobile Phase MeCN/H2O – 2/98%
Buffer H2SO4 or Formic Acid
Flow Rate 1.0 ml/min
Detection UV 275nm

Description

Class of Compounds Acid, Hydrophilic
Analyzing Compounds EDTA, NTA

 

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
EDTA (Ethylenediaminetetraacetic Acid)
Nitrilotriacetic acid
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Nitrilotriacetic acid on Newcrom R1 HPLC column

February 16, 2018
Separation of Nitrilotriacetic acid on Newcrom C18 HPLC column

Nitrilotriacetic acid can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Nitrilotriacetic acid
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.