Vitamin C

Vitamin C structural formula

CAS Number50-81-7
Molecular FormulaC6H8O6
Molecular Weight176.124
InChI KeyCIWBSHSKHKDKBQ-JLAZNSOCSA-N
LogP-1.85
Synonyms
  • L-Ascorbic acid
  • (5R)-5-[(1S)-1,2-Dihydroxyethyl]-3,4-dihydroxyfuran-2(5H)-one (non-preferred name)
  • 50-81-7
  • (+)-Ascorbic acid
  • 3-keto-L-Gulofuranolactone
  • 3-Oxo-L-gulofuranolactone
  • Acide ascorbique
  • acido ascorbico
  • Allercorb
  • Antiscorbic vitamin
  • Antiscorbutic vitamin
  • Ascoltin
  • Ascorbajen
  • ASCORBIC ACID, L-
  • Ascorbicap
  • ASCORBINSAEURE
  • Ascorbinsaure
  • Ascorbutina
  • Ascorell
  • Ascorin
  • Ascorteal
  • Ascorvit
  • Cantaxin
  • Catavin C
  • Cebicure
  • Cebion, γ-lactone
  • Cebione
  • Cegiolan
  • Ceglion
  • Celaskon
  • Cemagyl
  • Ce-Mi-Lin
  • Cenetone
  • Cergona
  • Cescorbat
  • Cetamid
  • Cetane-Caps TC
  • Cetemican
  • Cevalin
  • Cevatine
  • Cevimin
  • Ce-Vi-Sol
  • Cevital
  • Cevitamic acid
  • Cevitamin
  • Cevitan
  • Cevitex
  • Chewcee
  • Citrovit
  • Colascor
  • Concemin
  • C-Vimin
  • Davitamon C
  • Juvamine
  • Kangbingfeng
  • L-(+)-Ascorbic acid
  • L-3-Keto-threo-hexuronic acid lactone
  • Laroscorbine
  • Lemascorb
  • Liqui-Cee
  • L-Lyxoascorbic acid
  • L-threo-Ascorbic acid
  • L-threo-Hex-2-enonic acid, γ-lactone
  • L-Xyloascorbic acid
  • Neo-Valdrin
  • NSC 218455
  • NSC 33832
  • Planavit C
  • Proscorbin
  • Redoxon
  • Ronotec 100
  • Rovimix C
  • Rovimix C-EC
  • Scorbu C
  • Secorbate
  • Suncoat VC 40
  • Testascorbic
  • Vicelat
  • Viforcit
  • Viscorin
  • Viscorin 100M
  • Vitacee
  • Vitacimin
  • Vitacin
  • VITAMIN C
  • Vitamisin
  • Vitascorbol
  • Xyloascorbic acid, L-
  • L-Ascorbate
  • Caswell No. 061B
  • EINECS 200-066-2
  • FEMA No. 2109
  • Hex-2-enonic acid gamma-lactone, L-threo-
  • NCI-C54808
  • L-threo-Hex-2-enonic acid, gamma-lactone
  • Acidum ascorbicum
  • UNII-PQ6CK8PD0R
  • (+)-Sodium L-ascorbate
  • (+)-ascorbate
  • (5R)-5-[(1S)-1,2-dihydroxyethyl]-3,4-dihydroxy-2,5-dihydrofuran-2-one
  • Adenex
  • Allercorb
  • Arco-cee
  • Ascor-B.I.D.
  • Ascorb
  • Ascorbicab
  • Ascorbicin
  • Ascorbin
  • Ascorvit
  • C-Level
  • C-Long
  • C-Quin
  • C-Span
  • Cantan
  • Ce lent
  • Cebid
  • Cebion
  • Cecon
  • Cee-caps TD
  • Cee-vite
  • Ceklin
  • Celaskon
  • Celin
  • Cell C
  • Cemill
  • Cenolate
  • Cereon
  • Cetane
  • Cetane-caps TD
  • Cetebe
  • Cevex
  • Cevi-bid
  • Cevitamate
  • Cewin
  • Ciamin
  • Cipca
  • Citriscorb
  • Dora-C-500
  • Duoscorb
  • E 300
  • E-300
  • E300
  • Ferrous ascorbate
  • HiCee
  • Hybrin
  • Ido-C
  • Kyselina askorbova
  • L(+)-Ascorbate
  • L(+)-Ascorbic acid
  • L-(+)-Ascorbate
  • L-3-Ketothreohexuronic acid lactone
  • L-Lyxoascorbate
  • L-Xyloascorbate
  • Meri-c
  • Natrascorb
  • Natrascorb injectable
  • Redoxon
  • Ribena
  • Rontex 100
  • Roscorbic
  • Scorbacid
  • Scorbu-C
  • Sodascorbate
  • VASC
  • Vicin
  • Vicomin C
  • Viscorin 100M
  • Vitace
  • Xitix
  • acidum ascorbinicum
  • gamma-Lactone L-threo-Hex-2-enonate
  • gamma-Lactone L-threo-Hex-2-enonic acid
  • 1018124-03-2
  • 129940-97-2
  • 14536-17-5
  • 154170-90-8
  • 259133-78-3
  • 30208-61-8
  • 50976-75-5
  • 56172-55-5
  • 56533-05-2
  • 57304-74-2
  • 57606-40-3
  • 623158-95-2
  • 882690-91-7
  • 884381-69-5
  • 885512-24-3
  • 88845-26-5
  • 89924-69-6
  • 1428525-25-0
  • 1703051-92-6

Applications:

Separation of Vitamins in Mixed-Mode HPLC

2010-08-22


Separation of Vitamin B2 and Vitamin C is demonstrated on a Primesep D mixed-mode column. This column can be used for analysis of other hydrophobic or neutral vitamins and supplements.

Condition

Column Primesep D , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Acetic Acid
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Vitamins C (ascorbic acid) and B2 (riboflavin)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep D

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Vitamin B2 (Riboflavin)
Vitamin C

Application Detection:
UV Detection

HILIC Separation of Common Preservatives - Citric Acid, Ascorbic Acid and EDTA

2009-07-16

Citric acid, ascorbic acid, and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.

Condition

Column Obelisc N , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Acid, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Citric acid, ascorbic acid and EDTA
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Obelisc N

SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters  - Buffer concentration, Buffer pH, and Organic Modifier Concentration  - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Ascorbic Acid
EDTA (Ethylenediaminetetraacetic Acid)
Vitamin C

Application Detection:
ELSD/MS Detection

HPLC Separation of Vitamin C and Vitamin B2

2006-11-21

Vitamin C (ascorbic acid) and Vitamin B2 (riboflavin) are water-soluble vitamins that are not stored in the body and must be replaced each day. Primesep D easily resolves Vitamins C and B2 and the elution order can be reversed by adjusting acetonitrile and acetic acid concentrations in the mobile phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and acetic acid (AcOH) and UV detection at 250 nm.

Condition

Column Primesep D , 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Acetic Acid
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Vitamins C (ascorbic acid) and B2 (riboflavin)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep D

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Vitamin B2 (Riboflavin)
Vitamin C

Application Detection:
UV Detection

Separation and Retention of Vitamins in Mixed-Mode HPLC

2005-05-05

Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Both columns allow peak order reversal by simply changing the acetonitrile concentration in the mobile phase. Vitamin B2 is especially sensitive to acetonitrile concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA) or acetic acid (HOAc) with UV detection at 250 nm.

Condition

Column Primesep B, Primesep B2 , 3.2×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid, Acetic Acid
Flow Rate 0.5 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Vitamins C (ascorbic acid) and B2 (riboflavin)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep B

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options

Primesep B2

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Vitamin B2 (Riboflavin)
Vitamin C

Application Detection:
UV Detection

HPLC Separation of Two Vitamins: Different Polarity - Isocratic Methods

2004-05-13

Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.

Condition

Column Primesep B, Primesep B2 , 3.2×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid, Acetic Acid
Flow Rate 0.5 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Drug, Vitamin B₆, Hydrophobic, Ionizable
Analyzing Compounds Vitamins C (ascorbic acid) and B2 (riboflavin)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep B

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options

Primesep B2

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:

Ascorbic Acid
Vitamin B2 (Riboflavin)
Vitamin C

Application Detection:
UV Detection