| CAS Number | 50-81-7 |
|---|---|
| Molecular Formula | C6H8O6 |
| Molecular Weight | 176.124 |
| InChI Key | CIWBSHSKHKDKBQ-JLAZNSOCSA-N |
| LogP | -1.85 |
| Synonyms |
|
Applications:
Separation of Vitamins in Mixed-Mode HPLC
August 22, 2010
Separation of Vitamin B2 and Vitamin C is demonstrated on a Primesep D mixed-mode column. This column can be used for analysis of other hydrophobic or neutral vitamins and supplements.
| Column | Primesep D , 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Acetic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Column
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsVitamin C
HILIC Separation of Common Preservatives – Citric Acid, Ascorbic Acid and EDTA
July 16, 2009
Citric acid, ascorbic acid, and EDTA are commonly used in food and pharmaceutical industry as preservatives. These compounds are very polar in nature. They are weak organic acids with limited UV activity. Retention and separation is achieved on HILIC mixed-mode Obelisc N column. All three compounds are retained by combination of strong HILIC and strong anion-exchange mechanisms. Separation can be monitored by ELSD, LC/MS, UV or Corona CAD. In contrast to other HILIC column, Obelisc N has two ionizable groups basic and acidic which provide ion-exchange interaction in addition to hydrophilic interaction. This allows to use less acetonitrile for HILIC separation.
| Column | Obelisc N , 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmAc pH 4.0 |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Acid, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Citric acid, ascorbic acid and EDTA |
Application Column
Obelisc N
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsEDTA (Ethylenediaminetetraacetic Acid)
Vitamin C
HPLC Separation of Vitamin C and Vitamin B2
November 21, 2006
Vitamin C (ascorbic acid) and Vitamin B2 (riboflavin) are water-soluble vitamins that are not stored in the body and must be replaced each day. Primesep D easily resolves Vitamins C and B2 and the elution order can be reversed by adjusting acetonitrile and acetic acid concentrations in the mobile phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and acetic acid (AcOH) and UV detection at 250 nm.
| Column | Primesep D , 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Acetic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Column
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsVitamin C
HPLC Separation and Retention of Vitamins in Mixed-Mode on Primesep B2
May 5, 2005
Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Both columns allow peak order reversal by simply changing the acetonitrile concentration in the mobile phase. Vitamin B2 is especially sensitive to acetonitrile concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA) or acetic acid (HOAc) with UV detection at 250 nm.
| Column | Primesep B2, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid, Acetic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Column
Primesep B2
Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Vitamin C
HPLC Separation and Retention of Vitamins in Mixed-Mode on Primesep B
May 5, 2005
Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Both columns allow peak order reversal by simply changing the acetonitrile concentration in the mobile phase. Vitamin B2 is especially sensitive to acetonitrile concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either trifluoroacetic acid (TFA) or acetic acid (HOAc) with UV detection at 250 nm.
| Column | Primesep B, 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid, Acetic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Column
Primesep B
Column Diameter: 3.2 mm
Column Length: 50 mm
Particle Size: 5 µm
Pore Size: 100 A
Vitamin C
HPLC Separation of Two Vitamins: Different Polarity – Isocratic Methods
May 13, 2004
Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.
| Column | Primesep B, Primesep B2 , 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid, Acetic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsAscorbic Acid
Vitamin B2 (Riboflavin)
Vitamin C
