Primesep B and B2 columns separate Vitamins C (ascorbic acid) and B2 (riboflavin) with tunable selectivity. Peak order reversal is exhibited on these two columns with the same mobile phases due to their different polarity. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and either formic or acetic acid (HOAc) with UV detection at 250 nm.
| Column | Primesep B, Primesep B2 , 3.2×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid, Acetic Acid |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
| Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
Application Column
Primesep B
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options
Primesep B2
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsAscorbic Acid
Vitamin B2 (Riboflavin)
Vitamin C
