Separation of Vitamin B2 and Vitamin C is demonstrated on a Primesep D mixed-mode column. This column can be used for analysis of other hydrophobic or neutral vitamins and supplements.
Column | Primesep D , 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O |
Buffer | Acetic Acid |
Flow Rate | 1.0 ml/min |
Detection | UV, 250 nm |
Class of Compounds |
Drug, Vitamin B₆, Hydrophobic, Ionizable |
Analyzing Compounds | Vitamins C (ascorbic acid) and B2 (riboflavin) |
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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