HPLC Determination of EDTA, Phenoxyethanol and Methylparaben in Facial Cleanser on Newcrom BH

HPLC Method for EDTA (Ethylenediaminetetraacetic Acid), Methylparaben, Methylparaben sodium, 2-Phenoxyethanol on Newcrom BH by SIELC Technologies

 

High Performance Liquid Chromatography (HPLC) Method for Analysis of EDTA (Ethylenediaminetetraacetic Acid), Methylparaben, Methylparaben sodium, 2-Phenoxyethanol.

Ethylenediaminetetraacetic acid (EDTA) is a synthetic amino acid with the chemical formula C10H16N2O8. It is typically used in industry to sequester metal ions, which helps prevent change of colors in textiles and uneven bleaching in paper. Due to it being a chelator, it is also used to soften water during laundry, remove hydrogen sulfide from gas streams, as well as treat mercury and lead poisoning.

Phenoxyethanol is a synthetic compound with the chemical formula C8H10O2. It is said to have antimicrobial and preservative properties, leading to wide use of it in cosmetics, medicine, and biocides. It is considered safe in the US and Europe at limited concentrations.

Methylparaben, also known as Methyl 4-hydroxybenzoate, is a paraben with the chemical formula C8H8O3. It is used as a preservative in food, cosmetics, and pharmaceuticals as it is said to have antimicrobial and antifungal properties. It is considered safe for use in low concentrations, but it may cause irritation or contact dermatitis in rare cases and for those who are allergic.

You can find detailed UV spectra of EDTA + Fe complex and information about its various lambda maxima by visiting the following link.

You can find detailed UV spectra of Methylparaben and information about its various lambda maxima by visiting the following link.

EDTA (Ethylenediaminetetraacetic Acid), Methylparaben, Methylparaben sodium, 2-Phenoxyethanol can be retained and analyzed using the Newcrom BH stationary phase column. The analysis utilizes a gradient method with a simple mobile phase consisting of water and acetonitrile (MeCN) with a sulfuric acid buffer. Detection is performed using UV.

Condition

Column Newcrom BH, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile Phase Gradient MeCN – 2- 70%, 10 min
Buffer H2SO4 – 0.1%
Flow Rate 1.0 ml/min
Detection UV 260nm

Description

Class of Compounds Acid, Hydrophilic, Preservatives
Analyzing Compounds EDTA (Ethylenediaminetetraacetic Acid), Methylparaben, Methylparaben sodium, 2-Phenoxyethanol

 

Application Column

Newcrom BH

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
2-Phenoxyethanol
EDTA (Ethylenediaminetetraacetic Acid)
Methylparaben
Methylparaben sodium

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.