HPLC Retention of EDTA in Sulfated CD

EDTA, or Ethylenediaminetetraacetic acid, is widely used in textile, pulp/paper, food and pharmaceutical industries. It has application as chelating agent and preservative. EDTA molecule is very hydrophilic and contains two basic groups and four carboxylic groups. It has tendency to bind to metals, making analysis of EDTA by HPLC very challenging. EDTA is analyzed in the presence of copper sulfate as visualization agent on a Primesep SB column. The method can be used for EDTA determination in various mixtures and compositions using UV detector.


Column Primesep  SB , 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer MeSO3H with CuSO4
Flow Rate 1.0 ml/min
Detection UV 300 nm


Class of Compounds Acid
Analyzing Compounds EDTA, or Ethylenediaminetetraacetic acid

Application Analytes:
EDTA (Ethylenediaminetetraacetic Acid)