EDTA, or Ethylenediaminetetraacetic acid, is widely used in textile, pulp/paper, food and pharmaceutical industries. It has application as chelating agent and preservative. EDTA molecule is very hydrophilic and contains two basic groups and four carboxylic groups. It has tendency to bind to metals, making analysis of EDTA by HPLC very challenging. EDTA is analyzed in the presence of copper sulfate as visualization agent on a Primesep SB column. The method can be used for EDTA determination in various mixtures and compositions using UV detector.
| Column | Primesep SB , 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | MeSO3H with CuSO4 |
| Flow Rate | 1.0 ml/min |
| Detection | UV 300 nm |
| Class of Compounds |
Acid |
| Analyzing Compounds | EDTA, or Ethylenediaminetetraacetic acid |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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