Venlafaxine

Venlafaxine structural formula

CAS Number93413-69-5
Molecular FormulaC17H27NO2
Molecular Weight277.408
InChI KeyPNVNVHUZROJLTJ-UHFFFAOYSA-N
LogP2.38
Synonyms
  • Venlafaxine
  • 1-[2-(Dimethylamino)-1-(4-methoxyphenyl)ethyl]cyclohexan-1-ol
  • Cyclohexanol, 1-[2-(dimethylamino)-1-(4-methoxyphenyl)ethyl]-
  • 93413-69-5
  • Cyclohexanol, 1-[2-(dimethylamino)-1-(4-methoxyphenyl)ethyl]-
  • Venlafaxina
  • Venlafaxinum
  • UNII-GRZ5RCB1QG
  • 1-[2-(dimethylamino)-1-(4-methoxyphenyl)ethyl]cyclohexan-1-ol
  • Efectin
  • Efexor
  • Effexor
  • Elafax
  • Trevilor
  • Venlafaxin
  • Venlafexine

Applications:

HPLC Method for Analysis of Venlafaxine on Primesep 100 Column

July 30, 2025

HPLC Method for Venlafaxine on Primesep 100 by SIELC Technologies


High Performance Liquid Chromatography (HPLC) Method for Analysis of Venlafaxine

Venlafaxine is an organic compound with the molecular formula C17H27NO2.

Properties:
Appearance: Typically a white to off-white crystalline solid.

Molecular weight: ~277.4 g/mol

Solubility: Soluble in organic solvents.

Uses: A type of antidepressant. It is believed to work by increasing levels of serotonin and norepinephrine in the brain.

Venlafaxine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 200 nm.

ColumnPrimesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended
Mobile PhaseMeCN – 80%
BufferH2SO4 – 0.1%
DetectionUV 200 nm

Class of CompoundsDrug
Analyzing CompoundsVenlafaxine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended

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Application Analytes:
Venlafaxine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

UV-Vis Spectrum of Venlafaxine

July 30, 2025
UV-Vis Spectrum of Venlafaxine. Absorption Maxima: 196 nm, 226 nm, 276 nm.

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

Application Analytes:
Venlafaxine
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Venlafaxine on Newcrom R1 HPLC column

February 16, 2018
Separation of Venlafaxine on Newcrom C18 HPLC column

Venlafaxine can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Venlafaxine
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us before ordering the column as there may be a more suitable column alternative by either e-mail: support@sielc.com or by phone: 847-229-2629.