HPLC Method for Venlafaxine on Primesep 100 by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Venlafaxine
Venlafaxine is an organic compound with the molecular formula C17H27NO2.
Properties:
Appearance: Typically a white to off-white crystalline solid.
Molecular weight: ~277.4 g/mol
Solubility: Soluble in organic solvents.
Uses: A type of antidepressant. It is believed to work by increasing levels of serotonin and norepinephrine in the brain.
Venlafaxine can be retained and analyzed using the Primesep 100 stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and sulfuric acid. Detection is performed using UV at 200 nm.
Column | Primesep 100, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
Mobile Phase | MeCN – 80% |
Buffer | H2SO4 – 0.1% |
Detection | UV 200 nm |
Class of Compounds | Drug |
Analyzing Compounds | Venlafaxine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended