HPLC Chiral Separation of Venlafaxine Enantiomers on Cyralis AM Column

HPLC Chiral Method for Analysis of Venlafaxine Enantiomers on Cyralis AM Column by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of Venlafaxine.

Venlafaxine serotonin-norepinephrine reuptake inhibitor (SNRI) with the molecular formula C17H27NO2. It is used primarily as an antidepressant to treat major depressive disorder, generalized anxiety disorder, panic disorder, and social anxiety disorder It is recommended as a fist line treatment for post-traumatic stress disorder. It works through blocking the transporter “reuptake” proteins for neurotransmitters affecting mood. It selectively inhibits the serotonin transporter and blocks the norepinephrine transporter. You can find detailed UV spectra of Venlafaxine and information about its various lambda maxima by visiting the following link.

This application describes a normal-phase chiral HPLC method for the separation of Venlafaxine enantiomers using SIELC’s Cyralis AM chiral column. Cyralis AM is an amylose-based chiral stationary phase (CSP) designed for enantiomeric separations, optical purity testing, chiral impurity analysis, and stereoisomer characterization in pharmaceutical, chemical, agrochemical, and fine chemical applications.

Venlafaxine can be retained and analyzed using the Cyralis AM stationary phase column. The analysis utilizes an isocratic method with a mobile phase consisting of Hexane, Ethanol, and DEA. Detection is performed using UV.

Condition

ColumnCyralis AM, 4.6 x 250 mm, 5 µm, 100 A, normal phase
Mobile PhaseHexane/Ethanol/DEA
Flow Rate1.0 mL/min
DetectionUV 275nm
Resolution2.7

Description

Class of CompoundsDrug
Analyzing CompoundsVenlafaxine

Application Column

Cyralis AM

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: normal phase

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Application Analytes:
Venlafaxine
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.